Simultaneous and cost-effective determination of ethylene glycol and glycolic acid in human serum and urine for emergency toxicology by GC-MS
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25500419
DOI
10.1016/j.clinbiochem.2014.12.002
PII: S0009-9120(14)00785-1
Knihovny.cz E-resources
- Keywords
- Chloroformate, GC-MS, Glycolic acid, Glycols, Toxicology,
- MeSH
- Cost-Benefit Analysis * MeSH
- Time Factors MeSH
- Ethylene Glycol blood urine MeSH
- Glycolates blood urine MeSH
- Humans MeSH
- Emergencies MeSH
- Gas Chromatography-Mass Spectrometry economics methods MeSH
- Toxicology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ethylene Glycol MeSH
- glycolic acid MeSH Browser
- Glycolates MeSH
OBJECTIVES: A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. DESIGN AND METHODS: Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. RESULTS: The assay was proved to be quadratic in the range of 50 to 5000 mgL(-1) with adequate accuracy (96.3-105.8%) and precision (CV ≤ 8.9%). CONCLUSION: The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R(2) 0.9933 for ethylene glycol and 0.9943 for glycolic acid).
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