OBJECTIVES: A simple, cost-effective, and fast gas chromatography method with mass spectrometry detection (GC-MS) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. DESIGN AND METHODS: Successful derivatization of glycolic acid with isobutyl chloroformate was achieved directly in serum/urine while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. RESULTS: The assay was proved to be quadratic in the range of 50 to 5000 mgL(-1) with adequate accuracy (96.3-105.8%) and precision (CV ≤ 8.9%). CONCLUSION: The method was successfully applied to quantify the selected compounds in serum of patients from emergency units and the results correlated well with parallel GC-FID measurements (R(2) 0.9933 for ethylene glycol and 0.9943 for glycolic acid).

Institute of Forensic Medicine and Toxicology 1st Faculty of Medicine Charles University Prague and General University Hospital 121 08 Prague 2 Czech Republic

Institute of Forensic Medicine and Toxicology 1st Faculty of Medicine Charles University Prague and General University Hospital 121 08 Prague 2 Czech Republic; Charles University Prague Faculty of Science Department of Analytical Chemistry Albertov 6 128 43 Prague 2 Czech Republic

Citace poskytuje Crossref.org