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Molecular analysis of the tlyA gene in Campylobacter lari

Matsubara, Keiko
Author Matsubara, Keiko Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, 920-1181, Japan
Nakajima, Takuya
Author Nakajima, Takuya Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Sagamihara, Kanagawa, 252-5201, Japan
Moore, John E
Author Moore, John E Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast, BT9 7AD, UK School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, UK Centre for Infection and Immunity, Queen's University, Belfast, BT9 7AB, Northern Ireland, UK
Millar, Beverley C
Author Millar, Beverley C Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast, BT9 7AD, UK
Murayama, Tsugiya
Author Murayama, Tsugiya Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, 920-1181, Japan
Matsuda, Motoo
Author Matsuda, Motoo Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Sagamihara, Kanagawa, 252-5201, Japan. matsuda@azabu-u.ac.jp

Folia microbiologica. 2015 Nov ; 60 (6) : 505-14. [epub] 20150424

Folia Microbiol (Praha)
ISSN 1874-9356 | 0015-5632
Source

Language English Country United States Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid25906999

PubMed 25906999
DOI 10.1007/s12223-015-0389-8
PII: 10.1007/s12223-015-0389-8
Knihovny.cz E-resources

MeSH
Bacterial Proteins chemistry genetics MeSH
Campylobacter lari chemistry classification genetics isolation & purification MeSH
Phylogeny MeSH
Hemolysin Proteins chemistry genetics MeSH
Campylobacter Infections microbiology veterinary MeSH
Chickens microbiology MeSH
Environmental Microbiology MeSH
Bivalvia microbiology MeSH
Molecular Sequence Data MeSH
Open Reading Frames MeSH
Promoter Regions, Genetic MeSH
Birds microbiology MeSH
Amino Acid Sequence MeSH
Base Sequence MeSH
Sequence Alignment MeSH
Animals MeSH
Check Tag
Animals MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Names of Substances
Bacterial Proteins MeSH
Hemolysin Proteins MeSH

Full-length tlyA gene and its adjacent genetic loci from the urease-positive thermophilic Campylobacter (UPTC) CF89-12 [approximately 15,000 base pairs (bp) in length], as well as a reference strain Campylobacter lari RM2100 (approximately 9,000 bp), were analyzed. The possible open-reading frame of tlyA from UPTC CF89-12 was shown to have 720 bp with a calculated molecular mass of approximately 26.7 kDa. Using a primer pair designed in silico, a total of approximately 1.1 kbp consisting of putative promoter region, structural gene for tlyA, and its adjacent genetic loci were identified in all 17 C. lari isolates [n = 13 for UPTC; n = 4 for urease-negative (UN) C. lari]. Although sequence differences were demonstrated at approximately 20 loci within the 90 bp non-coding (NC) region, including the putative promoter structure candidates immediately upstream of the tlyA gene among the 18 isolates including C. lari RM2100, no sequence differences were identified within the NC region among the five UN C. lari isolates examined. A start codon ATG and a probable ribosome-binding site, AGGC(T)GG(A), for the tlyA gene were identified in all 18 isolates, including C. lari RM2100. The putative intrinsic ρ-independent transcriptional terminator structure candidate was also identified for the tlyA gene in both UPTC CF89-12 and C. lari RM2100. Additionally, the hemolysis assay was performed with some of the C. lari isolates. The tlyA gene nucleotide sequence data may possibly be useful for discrimination between UN C. lari and UPTC organisms, as well as for the differentiation among the four thermophilic Campylobacter species.

Centre for Infection and Immunity Queen's University Belfast BT9 7AB Northern Ireland UK

Department of Bacteriology Northern Ireland Public Health Laboratory Belfast City Hospital Belfast BT9 7AD UK

Faculty of Pharmaceutical Sciences Hokuriku University Kanazawa 920 1181 Japan

Laboratory of Molecular Biology Graduate School of Environmental Health Sciences Azabu University Sagamihara Kanagawa 252 5201 Japan

School of Biomedical Sciences University of Ulster Coleraine BT52 1SA UK

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