In chronic myeloid leukemia, the identification of individual BCR-ABL1 fusions is required for the development of personalized medicine approach for minimal residual disease monitoring at the DNA level. Next generation sequencing (NGS) of amplicons larger than 1000 bp simplified and accelerated a process of characterization of patient-specific BCR-ABL1 genomic fusions. NGS of large regions upstream and downstream the individual breakpoints in BCR and ABL1 genes, respectively, also provided information about the sequence variants such are single nucleotide polymorphisms.

CLIP Department of Paediatric Haematology and Oncology 2nd Faculty of Medicine Charles University Prague and University Hospital Motol Prague Czech Republic

Department of Experimental Diagnostic and Specialty Medicine Institute of Hematology L e A Seragnoli University of Bologna Bologna Italy

Institute of Clinical and Experimental Hematology of 1st Faculty of Medicine and Institute of Hematology and Blood Transfusion Charles University Prague Czech Republic

Institute of Hematology and Blood Transfusion Prague Czech Republic

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