Development and characterization of polyclonal peptide antibodies for the detection of Yellow fever virus proteins
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
26086983
DOI
10.1016/j.jviromet.2015.06.006
PII: S0166-0934(15)00222-0
Knihovny.cz E-resources
- Keywords
- Capsid protein, NS1 protein, Peptide antisera, Yellow fever virus,
- MeSH
- Antigens, Viral analysis immunology metabolism MeSH
- Cell Nucleus chemistry virology MeSH
- Chlorocebus aethiops MeSH
- Diagnostic Tests, Routine methods MeSH
- Fluorescent Antibody Technique MeSH
- Rabbits MeSH
- Guinea Pigs MeSH
- Peptides isolation & purification metabolism MeSH
- Antibodies, Viral immunology isolation & purification metabolism MeSH
- Sensitivity and Specificity MeSH
- Vero Cells MeSH
- Virology methods MeSH
- Viral Nonstructural Proteins analysis immunology metabolism MeSH
- Capsid Proteins analysis immunology metabolism MeSH
- Yellow fever virus immunology isolation & purification MeSH
- Blotting, Western MeSH
- Cross Reactions MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Guinea Pigs MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antigens, Viral MeSH
- NS1 protein, Flavivirus MeSH Browser
- Peptides MeSH
- Antibodies, Viral MeSH
- Viral Nonstructural Proteins MeSH
- Capsid Proteins MeSH
There is still a considerable need for development of new tools and methods detecting specific viral proteins for the diagnosis and pathogenesis study of the Yellow fever virus (YFV). This study aimed to develop and characterize polyclonal peptide antisera for detection of YFV-C and -NS1 proteins. The antisera were used further to investigate NS1 protein expression during YFV infection in mammalian cells. YFV target proteins were detected by all antisera in western blot and immunofluorescence assays. No cross-reactivity was observed with Dengue virus, West Nile virus, Tick-borne encephalitis virus and Japanese encephalitis virus. Nuclear localization of the YFV-C protein was demonstrated for the first time. Experiments investigating NS1 expression suggested a potential use of the YFV-NS1 antisera for development of diagnostic approaches targeting the secreted form of the NS1 protein. The antisera described in this study offer new possibilities for use in YFV research and for the development of novel diagnostic tests.
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