Development and characterization of polyclonal peptide antibodies for the detection of Yellow fever virus proteins
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26086983
DOI
10.1016/j.jviromet.2015.06.006
PII: S0166-0934(15)00222-0
Knihovny.cz E-zdroje
- Klíčová slova
- Capsid protein, NS1 protein, Peptide antisera, Yellow fever virus,
- MeSH
- antigeny virové analýza imunologie metabolismus MeSH
- buněčné jádro chemie virologie MeSH
- Cercopithecus aethiops MeSH
- diagnostické testy rutinní metody MeSH
- fluorescenční protilátková technika MeSH
- králíci MeSH
- morčata MeSH
- peptidy izolace a purifikace metabolismus MeSH
- protilátky virové imunologie izolace a purifikace metabolismus MeSH
- senzitivita a specificita MeSH
- Vero buňky MeSH
- virologie metody MeSH
- virové nestrukturální proteiny analýza imunologie metabolismus MeSH
- virové plášťové proteiny analýza imunologie metabolismus MeSH
- virus žluté zimnice imunologie izolace a purifikace MeSH
- western blotting MeSH
- zkřížené reakce MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- morčata MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny virové MeSH
- NS1 protein, Flavivirus MeSH Prohlížeč
- peptidy MeSH
- protilátky virové MeSH
- virové nestrukturální proteiny MeSH
- virové plášťové proteiny MeSH
There is still a considerable need for development of new tools and methods detecting specific viral proteins for the diagnosis and pathogenesis study of the Yellow fever virus (YFV). This study aimed to develop and characterize polyclonal peptide antisera for detection of YFV-C and -NS1 proteins. The antisera were used further to investigate NS1 protein expression during YFV infection in mammalian cells. YFV target proteins were detected by all antisera in western blot and immunofluorescence assays. No cross-reactivity was observed with Dengue virus, West Nile virus, Tick-borne encephalitis virus and Japanese encephalitis virus. Nuclear localization of the YFV-C protein was demonstrated for the first time. Experiments investigating NS1 expression suggested a potential use of the YFV-NS1 antisera for development of diagnostic approaches targeting the secreted form of the NS1 protein. The antisera described in this study offer new possibilities for use in YFV research and for the development of novel diagnostic tests.
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