Targeted neural differentiation of murine mesenchymal stem cells by a protocol simulating the inflammatory site of neural injury
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26118945
DOI
10.1002/term.2059
Knihovny.cz E-zdroje
- Klíčová slova
- adipose-derived mesenchymal stem cells, electrical stimulation, mouse, nanofibre scaffold, neural differentiation, neural injury,
- MeSH
- buněčná diferenciace * MeSH
- diferenciační antigeny biosyntéza MeSH
- mezenchymální kmenové buňky metabolismus patologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- nervová tkáň metabolismus patologie MeSH
- nervové kmenové buňky metabolismus patologie MeSH
- zánět metabolismus patologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- diferenciační antigeny MeSH
Damaged neural tissue is regenerated by neural stem cells (NSCs), which represent a rare and difficult-to-culture cell population. Therefore, alternative sources of stem cells are being tested to replace a shortage of NSCs. Here we show that mouse adipose tissue-derived mesenchymal stem cells (MSCs) can be effectively differentiated into cells expressing neuronal cell markers. The differentiation protocol, simulating the inflammatory site of neural injury, involved brain tissue extract, fibroblast growth factor, epidermal growth factor, supernatant from activated splenocytes and electrical stimulation under physiological conditions. MSCs differentiated using this protocol displayed neuronal cell morphology and expressed genes for neuronal cell markers, such as neurofilament light (Nf-L), medium (Nf-M) and heavy (Nf-H) polypeptides, synaptophysin (SYP), neural cell adhesion molecule (NCAM), glutamic acid decarboxylase (GAD), neuron-specific nuclear protein (NeuN), βIII-tubulin (Tubb3) and microtubule-associated protein 2 (Mtap2), which are absent (Nf-L, Nf-H, SYP, GAD, NeuN and Mtap2) or only slightly expressed (NCAM, Tubb3 and Nf-M) in undifferentiated cells. The differentiation was further enhanced when the cells were cultured on nanofibre scaffolds. The neural differentiation of MSCs, which was detected at the level of gene expression, was confirmed by positive immunostaining for Nf-L protein. The results thus show that the simulation of conditions in an injured neural tissue and inflammatory environment, supplemented with electrical stimulation under physiological conditions and cultivation of cells on a three-dimensional (3D) nanofibre scaffold, form an effective protocol for the differentiation of MSCs into cells with neuronal markers. Copyright © 2015 John Wiley & Sons, Ltd.
Faculty of Biomedical Engineering Czech Technical University Prague Kladno Czech Republic
Faculty of Science Charles University Prague Czech Republic
Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic
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