Intracellular Monitoring of AS1411 Aptamer by Time-Resolved Microspectrofluorimetry and Fluorescence Imaging
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26179074
DOI
10.1007/s10895-015-1612-3
PII: 10.1007/s10895-015-1612-3
Knihovny.cz E-zdroje
- Klíčová slova
- AS1411 aptamer, Aptamer, Fluorescence microscopy imaging, Intracellular fluorescence, Phase-modulation lifetime measurement,
- MeSH
- aptamery nukleotidové metabolismus MeSH
- časové faktory MeSH
- fluorescenční mikroskopie metody MeSH
- fluorescenční spektrometrie metody MeSH
- intracelulární prostor genetika metabolismus MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- oligodeoxyribonukleotidy genetika metabolismus MeSH
- sekvence nukleotidů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- AGRO 100 MeSH Prohlížeč
- aptamery nukleotidové MeSH
- oligodeoxyribonukleotidy MeSH
Time-resolved microspectrofluorimetry and fluorescence microscopy imaging-two complementary fluorescence techniques-provide important information about the intracellular distribution, level of uptake and binding/interactions inside living cell of the labeled molecule of interest. They were employed to monitor the "fate" of AS1411 aptamer labeled by ATTO 425 in human living cells. Confocal microspectrofluorimeter adapted for time-resolved intracellular fluorescence measurements by using a phase-modulation principle with homodyne data acquisition was employed to obtain emission spectra and to determine fluorescence lifetimes in U-87 MG tumor brain cells and Hs68 non-tumor foreskin cells. Acquired spectra from both the intracellular space and the reference solutions were treated to observe the aptamer localization and its interaction with biological structures inside the living cell. The emission spectra and the maximum emission wavelengths coming from the cells are practically identical, however significant lifetime lengthening was observed for tumor cell line in comparison to non-tumor one.
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