The aim of this work is to evaluate the effect three different storage temperatures (6, 23 and 40 °C) on the sterilized processed cheese quality during 24-month storage. Sterilized processed cheese (SPC) is a product with extended shelf life (up to 2 years). The samples of SPC were subjected to basic chemical analyses, i.e. pH-values, dry matter, fat, crude protein and ammonia content, and microbiological analyses, i.e. total number of microorganisms, number of coliforms, colony forming units of yeasts and/or moulds and spore-forming microorganisms. Furthermore, amino acid content (ion-exchange chromatography), protein profile (SDS-PAGE) and fat globules size (image analysis of microscopic technique) were monitored and sensory analysis (scale test and pair comparative test) was implemented, too. Increasing storage temperature and length evoked decrease of total amino acid content and protein nutrition value, increase of ammonia amount, protein changes, enlargement of fat globule size and deterioration of sensory properties of SPC. All the changes grew expressive with increasing storage temperature and time.

Department of Environmental Protection Engineering Faculty of Technology Tomas Bata University in Zlin Zlin The Czech Republic

Department of Food Technology Faculty of Technology Tomas Bata University in Zlin nam T G Masaryka 5555 760 01 Zlin The Czech Republic

Department of Vegetable Foodstuffs and Plant Production Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Brno The Czech Republic

Institute of Food Science and Biotechnology Faculty of Chemistry Brno University of Technology Brno The Czech Republic

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