Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26244853
DOI
10.1117/1.jbo.20.11.111206
PII: 2426326
Knihovny.cz E-resources
- MeSH
- Algorithms MeSH
- Holography methods MeSH
- Humans MeSH
- Microscopy methods MeSH
- Cell Line, Tumor MeSH
- Computer Simulation MeSH
- Image Processing, Computer-Assisted methods MeSH
- Scattering, Radiation MeSH
- Light MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect, which makes observation of samples possible also through scattering media. The paper describes theoretically and simulates numerically imaging of a two-dimensional object through a static scattering layer by means of CCHM, with the main focus on the quantitative phase imaging quality. The authors have investigated both strongly and weakly scattering media characterized by different amounts of ballistic and diffuse light. It is demonstrated that the phase information can be revealed also for the case of the static, strongly scattering layer. The dependence of the quality of imaging process on the spatial light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with a model phase object, as well as living carcinoma cells treated in an optically turbid emulsion.
References provided by Crossref.org
Holography microscopy as an artifact-free alternative to phase-contrast