Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion
Language English Country England, Great Britain Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30104699
PubMed Central
PMC6089916
DOI
10.1038/s41598-018-30408-7
PII: 10.1038/s41598-018-30408-7
Knihovny.cz E-resources
- MeSH
- Cell Membrane metabolism MeSH
- Cell Culture Techniques methods MeSH
- Fibrosarcoma diagnostic imaging pathology MeSH
- Holography instrumentation methods MeSH
- Intravital Microscopy instrumentation methods MeSH
- Neoplasm Invasiveness diagnostic imaging pathology MeSH
- Collagen metabolism MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Cell Movement * MeSH
- Pseudopodia metabolism MeSH
- Imaging, Three-Dimensional instrumentation methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Collagen MeSH
Observation and analysis of cancer cell behaviour in 3D environment is essential for full understanding of the mechanisms of cancer cell invasion. However, label-free imaging of live cells in 3D conditions is optically more challenging than in 2D. Quantitative phase imaging provided by coherence controlled holographic microscopy produces images with enhanced information compared to ordinary light microscopy and, due to inherent coherence gate effect, enables observation of live cancer cells' activity even in scattering milieu such as the 3D collagen matrix. Exploiting the dynamic phase differences method, we for the first time describe dynamics of differences in cell mass distribution in 3D migrating mesenchymal and amoeboid cancer cells, and also demonstrate that certain features are shared by both invasion modes. We found that amoeboid fibrosarcoma cells' membrane blebbing is enhanced upon constriction and is also occasionally present in mesenchymally invading cells around constricted nuclei. Further, we demonstrate that both leading protrusions and leading pseudopods of invading fibrosarcoma cells are defined by higher cell mass density. In addition, we directly document bundling of collagen fibres by protrusions of mesenchymal fibrosarcoma cells. Thus, such a non-invasive microscopy offers a novel insight into cellular events during 3D invasion.
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