Invadopodia and podosomes have been intensively studied because of their involvement in the degradation of extracellular matrix. As both structures have been studied mostly on thin matrices, their commonly reported shapes and characteristics may differ from those in vivo. To assess the morphology of invadopodia in a complex 3D environment, we observed invadopodial formation in cells grown on a dense matrix based on cell-free dermis. We have found that invadopodia differ in morphology when cells grown on the dermis-based matrix and thin substrates are compared. The cells grown on the dermis-based matrix display invadopodia which are formed by a thick protruding base rich in F-actin, phospho-paxillin, phospho-cortactin and phosphotyrosine signal, from which numerous thin filaments protrude into the matrix. The protruding filaments are composed of an F-actin core and are free of phospho-paxillin and phospho-cortactin but capped by phosphotyrosine signal. Furthermore, we found that a matrix-degrading activity is localized to the base of invadopodia and not along the matrix-penetrating protrusions. Our description of invadopodial structures on a dermis-based matrix should greatly aid the development of new criteria for the identification of invadopodia in vivo, and opens up the possibility of studying the invadopodia-related signaling in a more physiological environment.

Department of Cell Biology Faculty of Science Charles University Prague Prague Czech Republic

Citace poskytuje Crossref.org

Cytoplasmic Tail of MT1-MMP: A Hub of MT1-MMP Regulation and Function

Invadopodia Structure in 3D Environment Resolved by Near-Infrared Branding Protocol Combining Correlative Confocal and FIB-SEM Microscopy

High-throughput transcriptomic and proteomic profiling of mesenchymal-amoeboid transition in 3D collagen

Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion

Migrastatics-Anti-metastatic and Anti-invasion Drugs: Promises and Challenges

Cell polarity signaling in the plasticity of cancer cell invasiveness

Pragmatic medicine in solid cancer: a translational alternative to precision medicine

PKCα promotes the mesenchymal to amoeboid transition and increases cancer cell invasiveness

Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signaling

Drugs for solid cancer: the productivity crisis prompts a rethink

Invasive cells in animals and plants: searching for LECA machineries in later eukaryotic life

Tyrosine phosphorylation within the SH3 domain regulates CAS subcellular localization, cell migration, and invasiveness

The role of the tissue microenvironment in the regulation of cancer cell motility and invasion