Light microscopic morphometrics, ultrastructure, and molecular phylogeny of the putative pycnotrichid Ciliate, Buxtonella sulcata
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
26322499
DOI
10.1016/j.ejop.2015.06.003
PII: S0932-4739(15)00052-8
Knihovny.cz E-zdroje
- Klíčová slova
- Light microscopy, Morphometric data, Phylogeny, Scanning electron microscopy, Transmission electron microscopy, rDNA,
- MeSH
- Ciliophora klasifikace genetika ultrastruktura MeSH
- druhová specificita MeSH
- fylogeneze * MeSH
- geny rRNA genetika MeSH
- mezerníky ribozomální DNA genetika MeSH
- mikroskopie elektronová rastrovací MeSH
- molekulární sekvence - údaje MeSH
- protozoální DNA genetika MeSH
- skot MeSH
- transmisní elektronová mikroskopie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- mezerníky ribozomální DNA MeSH
- protozoální DNA MeSH
The ciliate, Buxtonella sulcata, was isolated from a bull cow near Tišnov, Czech Republic, and fixed for light (LM), scanning electron (SEM) and transmission electron microscopic (TEM) study. Presented here are the basic morphometrics from LM study, and the fine-structure of both somatic and vestibular ciliary, and other structures. While many morphological features are similar to ciliates belonging to the order Vestibuliferida, some differences have been discovered, and are presented here. Especially emphasized are the microtubular and fibrilar components of the basic kinetid structures for both somatic and vestibular regions of these protists. Also observed in both TEM and SEM samples were enigmatic membrane bulges at the base of many somatic cilia. These ciliates are seen to have abundant endocytoplasmic bacteria, as seen in LM and TEM. This evaluation of the ultrastructural morphology of B. sulcata from cattle is accompanied by detailed determination of its small subunit rRNA (SSU rRNA) gene sequence and also of internal transcribed spacers (ITS1-5.8rRNA-ITS2). All of these data will contribute to unravel the phylogenetic relationships of medically and veterinary important intestinal ciliates.
Citace poskytuje Crossref.org
GENBANK
KP016715, KP016716
