Development and validation of a FIA/UV-vis method for pK(a) determination of oxime based acetylcholinesterase reactivators
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Validation Study
PubMed
26386953
DOI
10.1016/j.jpba.2015.09.010
PII: S0731-7085(15)30151-5
Knihovny.cz E-resources
- Keywords
- FIA/UV–vis, Organophosphate, Oxime, Reactivator, UV–vis, pK(a),
- MeSH
- Acetylcholinesterase analysis chemistry MeSH
- Chemistry, Pharmaceutical methods standards MeSH
- Oximes analysis chemistry MeSH
- Cholinesterase Reactivators analysis chemistry MeSH
- Spectrophotometry, Ultraviolet methods standards MeSH
- Structure-Activity Relationship MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Validation Study MeSH
- Names of Substances
- Acetylcholinesterase MeSH
- Oximes MeSH
- Cholinesterase Reactivators MeSH
Acetylcholinesterase reactivators (oximes) are compounds used for antidotal treatment in case of organophosphorus poisoning. The dissociation constants (pK(a1)) of ten standard or promising acetylcholinesterase reactivators were determined by ultraviolet absorption spectrometry. Two methods of spectra measurement (UV-vis spectrometry, FIA/UV-vis) were applied and compared. The soft and hard models for calculation of pK(a1) values were performed. The pK(a1) values were recommended in the range 7.00-8.35, where at least 10% of oximate anion is available for organophosphate reactivation. All tested oximes were found to have pK(a1) in this range. The FIA/UV-vis method provided rapid sample throughput, low sample consumption, high sensitivity and precision compared to standard UV-vis method. The hard calculation model was proposed as more accurate for pK(a1) calculation.
J Pharm Biomed Anal. 2016 Feb 5;119:175 PubMed
References provided by Crossref.org
Brominated oxime nucleophiles are efficiently reactivating cholinesterases inhibited by nerve agents
