Identification and characterization of natural antibodies against tau protein in an intravenous immunoglobulin product
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26616881
DOI
10.1016/j.jneuroim.2015.10.017
PII: S0165-5728(15)30063-1
Knihovny.cz E-resources
- Keywords
- Alzheimer disease, Avidity, Immunoreactivity, Intravenous immunoglobulins, Natural antibodies, Tau protein,
- MeSH
- Epitopes chemistry MeSH
- Immunoglobulin G immunology isolation & purification MeSH
- Immunosorbent Techniques MeSH
- Immunoglobulins, Intravenous chemistry immunology MeSH
- Humans MeSH
- Molecular Weight MeSH
- Peptides immunology MeSH
- tau Proteins immunology MeSH
- Protein Structure, Tertiary MeSH
- Protein Binding immunology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Epitopes MeSH
- Immunoglobulin G MeSH
- Immunoglobulins, Intravenous MeSH
- Peptides MeSH
- tau Proteins MeSH
The latest therapeutic approaches to Alzheimer disease are using intravenous immunoglobulin (IVIG) products. Therefore, the detailed characterization of target-specific antibodies naturally occurring in IVIG products is beneficial. We have focused on characterization of antibodies isolated against tau protein, a biomarker of Alzheimer's disease, from Flebogamma IVIG product. The analysis of IgG subclass distribution indicated skewing toward IgG3 in anti-tau-enriched IgG fraction. The evaluation of their reactivity and avidity with several recombinant tau forms was performed by ELISA and blotting techniques. Truncated non-phosphorylated tau protein (amino acids 155-421) demonstrated the highest reactivity and avidity index. We provide the first detailed insight into the reactivity of isolated natural antibodies against tau protein.
Department of Biological and Biochemical Sciences University of Pardubice Pardubice Czech Republic
Department of Neurobiology AD Center National Institute of Mental Health Klecany Czech Republic
References provided by Crossref.org
Autoantibodies targeting neuronal proteins as biomarkers for neurodegenerative diseases
