Cryptosporidium parvum and Enterocytozoon bieneusi in American Mustangs and Chincoteague ponies
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
26688100
DOI
10.1016/j.exppara.2015.12.004
PII: S0014-4894(15)30068-0
Knihovny.cz E-zdroje
- Klíčová slova
- Cryptosporidium, Feral horses, ITS, Microsporidia, SSU, gp60,
- MeSH
- Cryptosporidium parvum klasifikace genetika izolace a purifikace MeSH
- divoká zvířata MeSH
- Enterocytozoon klasifikace genetika izolace a purifikace MeSH
- feces parazitologie MeSH
- fylogeneze MeSH
- genotyp MeSH
- glykoproteiny genetika MeSH
- koně MeSH
- kryptosporidióza epidemiologie parazitologie přenos MeSH
- lidé MeSH
- mezerníky ribozomální DNA chemie MeSH
- mikrosporidióza epidemiologie parazitologie veterinární MeSH
- nemoci koní epidemiologie parazitologie MeSH
- prevalence MeSH
- RNA ribozomální genetika MeSH
- zoonózy parazitologie přenos MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Geografické názvy
- Spojené státy americké epidemiologie MeSH
- Názvy látek
- glykoproteiny MeSH
- mezerníky ribozomální DNA MeSH
- RNA ribozomální MeSH
The prevalence of Cryptosporidium and microsporidia in feral horses, which have minimal contact with livestock and humans, is not currently known. We report the findings of a study on Cryptosporidium and microsporidia in 34 Mustangs and 50 Chincoteague ponies in the USA. Fecal samples were screened for presence of Cryptosporidium spp. by analysis of the small-subunit rRNA (SSU) and 60-kDa glycoprotein (gp60) genes, and Enterocytozoon bieneusi and Encephalitozoon spp. by analysis of the ribosomal internal transcribed spacer region (ITS). Cryptosporidium spp. and E. bieneusi were detected in 28/84 (33.3%) and 7/84 (8.3%) samples, respectively. Sequence analysis of SSU and ITS revealed the presence of Cryptosporidium parvum (n = 20) and E. bieneusi genotype horse 1 (n = 7), respectively. Subtyping of C. parvum isolates at the gp60 locus showed the presence of subtype IIaA17G2R1 in Mustangs and subtypes IIaA13G2R1 and IIaA15G2R1 in Chincoteague ponies. Enterocytozoon bieneusi genotype horse 1 was detected in Mustangs (n = 2) and Chincoteague ponies (n = 5). No Cryptosporidium or E. bieneusi positive animals had diarrhea. The finding that Mustangs and Chincoteague ponies are host to the zoonotic pathogen C. parvum suggests that their infrequent contact with humans and livestock is sufficient to maintain transmission; however, we should also consider the possibility that C. parvum is an established parasite of Mustangs and Chincoteague ponies that persists in these animals independently of contact with humans or livestock.
Chincoteague National Wildlife Refuge Chincoteague Island VA USA
Department of Animal and Rangeland Sciences Oregon State University Corvallis OR USA
Department of Veterinary and Microbiological Sciences North Dakota State University Fargo ND USA
Faculty of Economics University of South Bohemia in České Budějovice Czech Republic
Citace poskytuje Crossref.org
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