1H, 13C and 15N resonance assignments of human DCL-1 (CD302) extracellular domain
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26725057
DOI
10.1007/s12104-015-9664-5
PII: 10.1007/s12104-015-9664-5
Knihovny.cz E-resources
- Keywords
- C-type lectin like receptor, DCL-1 (CD302), Heteronuclear NMR spectroscopy, Protein NMR, Resonance assignment,
- MeSH
- Extracellular Space * MeSH
- Lectins, C-Type chemistry MeSH
- Humans MeSH
- Nuclear Magnetic Resonance, Biomolecular * MeSH
- Protein Domains MeSH
- Receptors, Cell Surface chemistry MeSH
- Protein Structure, Secondary MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CD302 protein, human MeSH Browser
- Lectins, C-Type MeSH
- Receptors, Cell Surface MeSH
DCL-1 (CD302) is a single-pass type one transmembrane protein which is predominantly expressed on myeloid cell lines. It possess the ability of endocytosis and is assumed to play a role in cell adhesion and migration. It has been also connected to several illnesses but more on the level of mRNA than on the protein expression level. More interestingly it is alternatively expressed in the form of a fusion protein with another single-pass type one transmembrane protein DEC205 (CD205) which is normally involved in antigen-uptake and endocytosis. The fusion protein has been assigned to have altered function compared to the wild type proteins. We have performed NMR structural analysis of the 16.2 kDa extracellular domain of DCL-1 to get a better insight onto this molecule. We have been able to assign nearly 97 % of resonance frequencies for the (15)N and (13)C labeled recombinant protein. The assignments have been deposited into Biological Magnetic Resonance Data Bank under the accession number 25802.
Department of Biochemistry Faculty of Science Charles University Prague Prague Czech Republic
Institute of Microbiology Academy of Sciences of the Czech Republic Prague Czech Republic
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