Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Mercury, microbial community, mobility, rhizobox, speciation,
- MeSH
- Bacteria metabolismus MeSH
- biodegradace MeSH
- chromatografie plynová MeSH
- fluorescenční spektrometrie MeSH
- látky znečišťující půdu analýza chemie MeSH
- methylortuťné sloučeniny analýza chemie MeSH
- monitorování životního prostředí metody MeSH
- půdní mikrobiologie * MeSH
- rtuť analýza chemie MeSH
- sloučeniny rtuti analýza chemie MeSH
- spektrofotometrie atomová MeSH
- znečištění životního prostředí analýza MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- látky znečišťující půdu MeSH
- methylortuťné sloučeniny MeSH
- rtuť MeSH
- sloučeniny rtuti MeSH
The precise characterization of the behavior of individual microorganisms in the presence of increased mercury contents in soil is necessary for better elucidation of the fate of mercury in the soil environment. In our investigation, resistant bacterial strains isolated from two mercury contaminated soils, represented by Paenibacillus alginolyticus, Burkholderia glathei, Burkholderia sp., and Pseudomonas sp., were used. Two differently contaminated soils (0.5 and 7 mg kg(-1) total mercury) were chosen. Preliminary soil analysis showed the presence of methylmercury and phenylmercury with the higher soil mercury level. Modified rhizobox experiments were performed to assess the ability of mercury accumulating strains to deplete the mobile and mobilizable mercury portions in the soil by modification; microbial agar cultures were used rather than the plant root zone. A sequential extraction procedure was performed to release the following mercury fractions: water soluble, extracted in acidic conditions, bound to humic substances, elemental, and bound to complexes, HgS and residual. Inductively coupled plasma mass spectrometry (ICP-MS) and a single-purpose atomic absorption spectrometer (AMA-254) were applied for mercury determination in the samples and extracts. Gas chromatography coupled to atomic fluorescence spectrometry (GC-AFS) was used for the determination of organomercury compounds. The analysis of the microbial community at the end of the experiment showed a 42% abundance of Paenibacillus sp. followed by Acetivibrio sp., Brevibacillus sp., Cohnella sp., Lysinibacillus sp., and Clostridium sp. not exceeding 2% abundance. The results suggest importance of Paenibacillus sp. in Hg transformation processes. This genus should be tested for potential bioremediation use in further research.
b Institute of Microbiology Vídeňská Prague Czech Republic
c Atomic Absorption Spectrometry Laboratory Institute of Chemical Technology Prague Czech Republic
Department of Chemistry Faculty of Science Masaryk University Brno Czech Republic
e Institute of Analytical Chemistry Academy of Sciences of the Czech Republic Brno Czech Republic
f Department of Public Health Faculty of Medicine Masaryk University Brno Czech Republic
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