Fungal Communities in Soils: Soil Organic Matter Degradation
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Cellulose, Metagenomics, Metatranscriptomics, Microbial communities, Organic matter decomposition, Soil ecology, Stable isotope probing,
- MeSH
- Cellulose metabolism MeSH
- DNA, Fungal genetics MeSH
- Phylogeny MeSH
- Fungi MeSH
- Isotope Labeling methods MeSH
- Metagenomics * MeSH
- Soil Microbiology * MeSH
- Gene Expression Regulation, Fungal MeSH
- Substrate Specificity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cellulose MeSH
- DNA, Fungal MeSH
Stable isotope probing (SIP) provides the opportunity to label decomposer microorganisms that build their biomass on a specific substrate. In combination with high-throughput sequencing, SIP allows for the identification of fungal community members involved in a particular decomposition process. Further information can be gained through gene-targeted metagenomics and metatranscriptomics, opening the possibility to describe the pool of genes catalyzing specific decomposition reactions in situ and to identify the diversity of genes that are expressed. When combined with gene descriptions of fungal isolates from the same environment, specific biochemical reactions involved in decomposition can be linked to individual fungal taxa. Here we describe the use of these methods to explore the cellulolytic fungal community in forest litter and soil.
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