Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26847870
DOI
10.1002/pros.23159
Knihovny.cz E-zdroje
- Klíčová slova
- cancer metabolism, dimethylglycine, folate, glycine, prostate cancer, sarcosine pathway,
- MeSH
- aminokyseliny farmakologie MeSH
- buněčné linie MeSH
- glycin-N-methyltransferasa genetika metabolismus MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádory prostaty metabolismus patologie MeSH
- pohyb buněk účinky léků MeSH
- prostata účinky léků metabolismus patologie MeSH
- sarkosin metabolismus MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aminokyseliny MeSH
- glycin-N-methyltransferasa MeSH
- sarkosin MeSH
BACKGROUND: Sarcosine (N-methylglycine) was previously delineated as a substantial oncometabolite of prostate cancer (PCa) and its metabolism seems to be significantly involved in PCa development and behavior. METHODS: We focused on investigation whether the exposure of prostate cells (PNT1A, 22Rv1, and PC-3) to sarcosine-related amino acids (glycine, dimethylglycine, and sarcosine) affects their aggressiveness (cell mobility and division rates, using real-time cell based assay). The effect of supplementation on expression of glycine-N-methyltransferase (GNMT) mRNA was examined using qRT-PCR. Finally, post-treatment amino acids patterns were determined with consequent statistical processing using the Ward's method, factorial ANOVA and principal component analysis (P < 0.05). RESULTS: The highest migration induced sarcosine and glycine in metastatic PC-3 cells (a decrease in relative free area about 53% and 73%). The highest cell division was achieved after treatment of 22Rv1 and PC-3 cells with sarcosine (time required for division decreased by 65% or 45%, when compared to untreated cells). qRT-PCR revealed also significant effects on expression of GNMT. Finally, amino acid profiling shown specific amino acid patterns for each cell line. In both, treated and untreated PC-3 cells significantly higher levels of serine, glutamic acid, and aspartate, linked with prostate cancer progression were found. CONCLUSIONS: Sarcosine-related amino acids can exceptionally affect the behavior of benign and malignant prostate cells.
Faculty of Medicine Department of Physiology Masaryk University Brno Czech Republic European Union
Faculty of Science Department of Biochemistry Charles University Czech Republic European Union
Citace poskytuje Crossref.org
