Electrochemical sensing of etoposide using carbon quantum dot modified glassy carbon electrode
Language English Country Great Britain, England Media print
Document type Journal Article
PubMed
26882954
DOI
10.1039/c5an02476e
Knihovny.cz E-resources
- MeSH
- Electrochemistry instrumentation methods MeSH
- Electrodes MeSH
- Etoposide analysis chemistry pharmacology MeSH
- Quantum Dots chemistry MeSH
- Humans MeSH
- Limit of Detection MeSH
- Cell Line, Tumor MeSH
- Povidone chemistry MeSH
- Glass chemistry MeSH
- Carbon chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Etoposide MeSH
- Povidone MeSH
- Carbon MeSH
In this study, enhancement of the electrochemical signals of etoposide (ETO) measured by differential pulse voltammetry (DPV) by modifying a glassy carbon electrode (GCE) with carbon quantum dots (CQDs) is demonstrated. In comparison with a bare GCE, the modified GCE exhibited a higher sensitivity towards electrochemical detection of ETO. The lowest limit of detection was observed to be 5 nM ETO. Furthermore, scanning electron microscopy (SEM), fluorescence microscopy (FM), and electrochemical impedance spectroscopy (EIS) were employed for the further study of the working electrode surface after the modification with CQDs. Finally, the GCE modified with CQDs under optimized conditions was used to analyse real samples of ETO in the prostate cancer cell line PC3. After different incubation times (1, 3, 6, 9, 12, 18 and 24 h), these samples were then prepared prior to electrochemical detection by the GCE modified with CQDs. High performance liquid chromatography with an electrochemical detection method was employed to verify the results from the GCE modified with CQDs.
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