Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
26883261
DOI
10.1208/s12249-016-0500-8
PII: 10.1208/s12249-016-0500-8
Knihovny.cz E-resources
- Keywords
- PCL, compatibility study, composites, electrospinning, skin tissue engineering,
- MeSH
- Biocompatible Materials chemistry MeSH
- Cell Adhesion physiology MeSH
- Fibroblasts chemistry MeSH
- Photoelectron Spectroscopy methods MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Collagen chemistry MeSH
- Skin chemistry MeSH
- Nanofibers chemistry MeSH
- Polyesters chemistry MeSH
- Surface Properties MeSH
- Titanium chemistry MeSH
- Tissue Engineering methods MeSH
- Tissue Scaffolds MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biocompatible Materials MeSH
- Collagen MeSH
- polycaprolactone MeSH Browser
- Polyesters MeSH
- Titanium MeSH
- titanium dioxide MeSH Browser
In this study, biodegradable poly(ε-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.
References provided by Crossref.org
