Expression of microRNAs miR21, miR146a, and miR155 in tuberous sclerosis complex cortical tubers and their regulation in human astrocytes and SEGA-derived cell cultures
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
J 3499
Austrian Science Fund FWF - Austria
PubMed
27014996
DOI
10.1002/glia.22983
Knihovny.cz E-resources
- Keywords
- astrocytes, cultures, inflammation, microRNA, subependymal giant cell astrocytoma, tuberous sclerosis complex,
- MeSH
- Astrocytoma metabolism pathology MeSH
- Astrocytes metabolism MeSH
- Cell Culture Techniques MeSH
- Child MeSH
- Adult MeSH
- Infant MeSH
- Cells, Cultured MeSH
- Middle Aged MeSH
- Humans MeSH
- MicroRNAs metabolism MeSH
- Adolescent MeSH
- Young Adult MeSH
- Brain metabolism MeSH
- Neurons metabolism MeSH
- Child, Preschool MeSH
- Signal Transduction physiology MeSH
- Tuberous Sclerosis metabolism MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Child, Preschool MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- MicroRNAs MeSH
- MIRN146 microRNA, human MeSH Browser
- MIRN155 microRNA, human MeSH Browser
- MIRN21 microRNA, human MeSH Browser
Tuberous sclerosis complex (TSC) is a genetic disease presenting with multiple neurological symptoms including epilepsy, mental retardation, and autism. Abnormal activation of various inflammatory pathways has been observed in astrocytes in brain lesions associated with TSC. Increasing evidence supports the involvement of microRNAs in the regulation of astrocyte-mediated inflammatory response. To study the role of inflammation-related microRNAs in TSC, we employed real-time PCR and in situ hybridization to characterize the expression of miR21, miR146a, and miR155 in TSC lesions (cortical tubers and subependymal giant cell astrocytomas, SEGAs). We observed an increased expression of miR21, miR146a, and miR155 in TSC tubers compared with control and perituberal brain tissue. Expression was localized in dysmorphic neurons, giant cells, and reactive astrocytes and positively correlated with IL-1β expression. In addition, cultured human astrocytes and SEGA-derived cell cultures were used to study the regulation of the expression of these miRNAs in response to the proinflammatory cytokine IL-1β and to evaluate the effects of overexpression or knockdown of miR21, miR146a, and miR155 on inflammatory signaling. IL-1β stimulation of cultured glial cells strongly induced intracellular miR21, miR146a, and miR155 expression, as well as miR146a extracellular release. IL-1β signaling was differentially modulated by overexpression of miR155 or miR146a, which resulted in pro- or anti-inflammatory effects, respectively. This study provides supportive evidence that inflammation-related microRNAs play a role in TSC. In particular, miR146a and miR155 appear to be key players in the regulation of astrocyte-mediated inflammatory response, with miR146a as most interesting anti-inflammatory therapeutic candidate.
Department of Child Neurology Medical University of Warsaw Warsaw Poland
Department of Neurology and Epileptology Children's Memorial Health Institute Warsaw Poland
Department of Neurosurgery Anna Meyer Children's Hospital Florence Italy
Department of Pathology Academic Medical Center University of Amsterdam Amsterdam the Netherlands
Department of Pathology University Medical Center Utrecht Utrecht the Netherlands
Department of Pediatric Neurology University Medical Center Utrecht Utrecht The Netherlands
Department of Pediatrics Medical University of Vienna Vienna Austria
Pathology Unit Anna Meyer Children's Hospital Florence Italy
References provided by Crossref.org
The coding and non-coding transcriptional landscape of subependymal giant cell astrocytomas
