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Photoleucine Survives Backbone Cleavage by Electron Transfer Dissociation. A Near-UV Photodissociation and Infrared Multiphoton Dissociation Action Spectroscopy Study

Shaffer, Christopher J
Author Shaffer, Christopher J Department of Chemistry, University of Washington, Seattle, WA, 98195-1700, USA
Martens, Jonathan
Author Martens, Jonathan FELIX Laboratory, Radboud University Nijmegen, Institute for Molecules and Materials, Toernooiveld 7c, 6525ED, Nijmegen, The Netherlands
Marek, Aleš
Author Marek, Aleš Department of Chemistry, University of Washington, Seattle, WA, 98195-1700, USA Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Oomens, Jos
Author Oomens, Jos FELIX Laboratory, Radboud University Nijmegen, Institute for Molecules and Materials, Toernooiveld 7c, 6525ED, Nijmegen, The Netherlands van't Hoff Institute for Molecular Sciences, University of Amsterdam, 1098XH, Amsterdam, Science Park 908, The Netherlands
Tureček, František
Author Tureček, František Department of Chemistry, University of Washington, Seattle, WA, 98195-1700, USA. turecek@chem.washington.edu

Journal of the American Society for Mass Spectrometry. 2016 Jul ; 27 (7) : 1176-85. [epub] 20160408

J Am Soc Mass Spectrom
ISSN 1879-1123 | 1044-0305
Source

Status PubMed-not-MEDLINE Language English Country United States Media print-electronic

Document type Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid27059977

PubMed 27059977
DOI 10.1007/s13361-016-1390-4
PII: 10.1007/s13361-016-1390-4
Knihovny.cz E-resources

Keywords
Electron transfer dissociation, Infrared multiphoton dissociation action spectroscopy, Near-UV photodissociation, Peptide ions, Photoleucine label,
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Research Support, U.S. Gov't, Non-P.H.S. MeSH

We report a combined experimental and computational study aimed at elucidating the structure of N-terminal fragment ions of the c type produced by electron transfer dissociation of photo-leucine (L*) peptide ions GL*GGKX. The c 4 ion from GL*GGK is found to retain an intact diazirine ring that undergoes selective photodissociation at 355 nm, followed by backbone cleavage. Infrared multiphoton dissociation action spectra point to the absence in the c 4 ion of a diazoalkane group that could be produced by thermal isomerization of vibrationally hot ions. The c 4 ion from ETD of GL*GGK is assigned an amide structure by a close match of the IRMPD action spectrum and calculated IR absorption. The energetics and kinetics of c 4 ion dissociations are discussed. Graphical Abstract ᅟ.

Department of Chemistry University of Washington Seattle WA 98195 1700 USA

FELIX Laboratory Radboud University Nijmegen Institute for Molecules and Materials Toernooiveld 7c 6525ED Nijmegen The Netherlands

Institute of Organic Chemistry and Biochemistry Academy of Sciences of the Czech Republic Prague Czech Republic

van't Hoff Institute for Molecular Sciences University of Amsterdam 1098XH Amsterdam Science Park 908 The Netherlands

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