An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27106696
DOI
10.1007/s12223-016-0459-6
PII: 10.1007/s12223-016-0459-6
Knihovny.cz E-zdroje
- MeSH
- antigeny bakteriální analýza MeSH
- bakteriální adheze MeSH
- bakteriální adheziny analýza MeSH
- biofilmy růst a vývoj MeSH
- imunoanalýza metody MeSH
- polystyreny MeSH
- protilátky bakteriální metabolismus MeSH
- Staphylococcus aureus chemie fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny bakteriální MeSH
- bakteriální adheziny MeSH
- polystyreny MeSH
- protilátky bakteriální MeSH
Attachment of bacterial pathogens to the niche tissue in the host is the first step in biofilm formation leading to colonization and establishment of infection in the host. While the most common method used for determining the potential role of a bacterial antigen in biofilm formation has been demonstration of loss of this property using specific knockout mutants, it is an expensive and a laborious procedure. This study describes an alternative immunological assay for identification of attachment antigens of Staphylococcus aureus, potentially important in the development of an effective vaccine against infections caused by this pathogen. The method is based upon the concept of inhibition of attachment of S. aureus to PEGs coated with virulence antigen-specific antibodies. Antibodies used for validation of this assay were specific for ClfA, FnBPA, SdrD, PNAG and α-toxin, accredited biofilm-associated antigens of S. aureus.
Department of Cell Biology Albert Einstein College of Medicine Bronx 10461 NY USA
Department of Medicine Albert Einstein College of Medicine Bronx 10461 NY USA
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