Autocrine effects of transgenic resistin reduce palmitate and glucose oxidation in brown adipose tissue
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27113533
DOI
10.1152/physiolgenomics.00122.2015
PII: physiolgenomics.00122.2015
Knihovny.cz E-resources
- Keywords
- autocrine, brown adipose tissue, resistin, spontaneously hypertensive rat, transgenic,
- MeSH
- Autocrine Communication genetics physiology MeSH
- Glucose metabolism MeSH
- Adipose Tissue, Brown metabolism physiology MeSH
- Insulin metabolism MeSH
- Insulin Resistance physiology MeSH
- Muscle, Skeletal metabolism physiology MeSH
- Rats MeSH
- Fatty Acids, Nonesterified metabolism MeSH
- Lipid Metabolism physiology MeSH
- Mitochondria genetics physiology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Obesity metabolism physiopathology MeSH
- Oxidation-Reduction MeSH
- Palmitates metabolism MeSH
- Rats, Inbred SHR MeSH
- Rats, Transgenic MeSH
- Resistin genetics MeSH
- Transcriptome genetics MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Glucose MeSH
- Insulin MeSH
- Fatty Acids, Nonesterified MeSH
- Palmitates MeSH
- Resistin MeSH
Resistin has been originally identified as an adipokine that links obesity to insulin resistance in mice. In our previous studies in spontaneously hypertensive rats (SHR) expressing a nonsecreted form of mouse resistin (Retn) transgene specifically in adipose tissue (SHR-Retn), we have observed an increased lipolysis and serum free fatty acids, ectopic fat accumulation in muscles, and insulin resistance. Recently, brown adipose tissue (BAT) has been suggested to play an important role in the pathogenesis of metabolic disturbances. In the current study, we have analyzed autocrine effects of transgenic resistin on BAT glucose and lipid metabolism and mitochondrial function in the SHR-Retn vs. nontransgenic SHR controls. We observed that interscapular BAT isolated from SHR-Retn transgenic rats compared with SHR controls showed a lower relative weight (0.71 ± 0.05 vs. 0.91 ± 0.08 g/100 g body wt, P < 0.05), significantly reduced both basal and insulin stimulated incorporation of palmitate into BAT lipids (658 ± 50 vs. 856 ± 45 and 864 ± 47 vs. 1,086 ± 35 nmol/g/2 h, P ≤ 0.01, respectively), and significantly decreased palmitate oxidation (37.6 ± 4.5 vs. 57 ± 4.1 nmol/g/2 h, P = 0.007) and glucose oxidation (277 ± 34 vs. 458 ± 38 nmol/g/2 h, P = 0.001). In addition, in vivo microPET imaging revealed significantly reduced (18)F-FDG uptake in BAT induced by exposure to cold in SHR-Retn vs. control SHR (232 ± 19 vs. 334 ± 22 kBq/ml, P < 0.05). Gene expression profiles in BAT identified differentially expressed genes involved in skeletal muscle and connective tissue development, inflammation and MAPK and insulin signaling. These results provide evidence that autocrine effects of resistin attenuate differentiation and activity of BAT and thus may play a role in the pathogenesis of insulin resistance in the rat.
Institute for Clinical and Experimental Medicine Prague Czech Republic
Institute of Molecular Genetics Czech Academy of Sciences Prague Czech Republic;
Institute of Physiology Czech Academy of Sciences Prague Czech Republic;
References provided by Crossref.org
