Scrape Loading/Dye Transfer Assay
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
Grantová podpora
R01 ES013268
NIEHS NIH HHS - United States
- Klíčová slova
- Dye coupling, Dye transfer, Ex vivo assessment, Gap junctional intercellular communication assessment, High throughput, In vitro assay, Incision loading, Lucifer Yellow, Scalpel loading, Scrape loading, Tracers,
- MeSH
- dextrany metabolismus MeSH
- fluorescenční barviva metabolismus MeSH
- fluorescenční mikroskopie metody MeSH
- isochinoliny metabolismus MeSH
- kultivované buňky MeSH
- mezerový spoj metabolismus ultrastruktura MeSH
- mezibuněčná komunikace * MeSH
- myši MeSH
- rhodaminy metabolismus MeSH
- Sertoliho buňky MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- dextrany MeSH
- fluorescenční barviva MeSH
- isochinoliny MeSH
- lucifer yellow MeSH Prohlížeč
- rhodamine dextran MeSH Prohlížeč
- rhodaminy MeSH
The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs.
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