Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
27216121
DOI
10.1017/s0007485316000377
PII: S0007485316000377
Knihovny.cz E-resources
- Keywords
- Liparus glabrirostris, allopatric speciation, mitochondrial DNA, nuclear elongation factor 1α,
- MeSH
- Ecosystem * MeSH
- Phylogeny MeSH
- Phylogeography MeSH
- Genetic Variation MeSH
- Haplotypes MeSH
- DNA, Mitochondrial chemistry MeSH
- Weevils anatomy & histology genetics MeSH
- Sequence Analysis, DNA MeSH
- Social Isolation MeSH
- Genetic Speciation MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Europe MeSH
- Names of Substances
- DNA, Mitochondrial MeSH
Populations of Liparus glabrirostris (Curculionidae: Molytinae), a weevil inhabiting higher altitudes of Central Europe, were sampled from 24 localities in the Alps and Carpathian Mountains, and the geographical structuring of genetic variation was analyzed. Comparison of the concatenated mitochondrial cytochrome oxidase subunit I and subunit II sequences revealed consistent genetic divergence between the populations of L. glabrirostris from different mountain ranges. In phylogenetic analysis using maximum parsimony and median-joining networks, concatenated mitochondrial haplotypes from the Alps and Carpathians clustered as separate lineages, with high bootstrap support. Substantial genetic distances determined between the separated groups ranged from 2.6 to 3.0%, with divergence estimated to have initiated approximately 0.85-0.98 million years ago. The nuclear elongation factor 1α gene was additionally amplified and haplotype analysis showed very low evolutionary divergence (0.2%), with separate clustering as well. The observed divergence suggests that the populations have been isolated for a long time, as a consequence of environmental changes resulting in varying fragmentation of habitats in the Alps and Carpathians, interrupting genetic exchange events and altering the genetic structure of L. glabrirostris populations. On the other hand, comparison of morphological characteristics showed no differences to confirm genetically well differentiated groups of populations. A polymerase chain reaction and restriction fragment length polymorphism-based method was therefore developed to discriminate between the Alpine and Carpathian lineages.
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