DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
27232140
DOI
10.1111/jvec.12209
Knihovny.cz E-zdroje
- Klíčová slova
- Borrelia burgdorferi, Flagellin B, Lyme disease, OspC, Tick,
- MeSH
- antigeny bakteriální genetika MeSH
- Borrelia burgdorferi komplex klasifikace MeSH
- DNA bakterií genetika MeSH
- flagelin genetika MeSH
- fylogeneze MeSH
- klíště mikrobiologie MeSH
- lymeská nemoc MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- proteiny vnější bakteriální membrány genetika MeSH
- sérotypizace * MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- antigeny bakteriální MeSH
- DNA bakterií MeSH
- flaB1 protein, bacteria MeSH Prohlížeč
- flagelin MeSH
- OspC protein MeSH Prohlížeč
- proteiny vnější bakteriální membrány MeSH
Two different genetic loci, flaB and ospC, were employed to assign genospecies and OspC phylogenetic type to 18 strains isolated from ticks collected in Pisárky, a suburban park in the city of Brno, Czech Republic. The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii, and B. valaisiana). Three samples from the collection contained more than one genospecies. In the other 15 strains, nucleotide sequences of flaB and ospC were determined. The following phylogenetic analysis assigned 12 isolates to genospecies B. garinii and three to B. afzelii. These isolates were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, and G5 (B. garinii) and A3 and A8 (B. afzelii).
Citace poskytuje Crossref.org
Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold
