DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic)
Language English Country United States Media print
Document type Journal Article
PubMed
27232140
DOI
10.1111/jvec.12209
Knihovny.cz E-resources
- Keywords
- Borrelia burgdorferi, Flagellin B, Lyme disease, OspC, Tick,
- MeSH
- Antigens, Bacterial genetics MeSH
- Borrelia burgdorferi Group classification MeSH
- DNA, Bacterial genetics MeSH
- Flagellin genetics MeSH
- Phylogeny MeSH
- Ixodes microbiology MeSH
- Lyme Disease MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Bacterial Outer Membrane Proteins genetics MeSH
- Serotyping * MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Antigens, Bacterial MeSH
- DNA, Bacterial MeSH
- flaB1 protein, bacteria MeSH Browser
- Flagellin MeSH
- OspC protein MeSH Browser
- Bacterial Outer Membrane Proteins MeSH
Two different genetic loci, flaB and ospC, were employed to assign genospecies and OspC phylogenetic type to 18 strains isolated from ticks collected in Pisárky, a suburban park in the city of Brno, Czech Republic. The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii, and B. valaisiana). Three samples from the collection contained more than one genospecies. In the other 15 strains, nucleotide sequences of flaB and ospC were determined. The following phylogenetic analysis assigned 12 isolates to genospecies B. garinii and three to B. afzelii. These isolates were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, and G5 (B. garinii) and A3 and A8 (B. afzelii).
References provided by Crossref.org
Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold
