While the pathogenic role of dicarbonyl stress and accelerated formation of advanced glycation end products (AGEs) to glucose intolerance and to the development of diabetic complications is well established, little is known about these processes in gestational diabetes mellitus (GDM), a condition pathogenically quite similar to type 2 diabetes. The aims of the present study were (i) to determine plasma thiamine and erythrocyte thiamine diphosphate (TDP) and transketolase (TKT) activity in pregnant women with and without GDM, (ii) to assess relationships between thiamine metabolism parameters and selected clinical, biochemical and anthropometric characteristics and, finally, (iii) to analyse relationship between variability in the genes involved in the regulation of transmembrane thiamine transport (i.e. SLC19A2 and SLC19A3) and relevant parameters of thiamine metabolism. We found significantly lower plasma BMI adjusted thiamine in women with GDM (P = 0.002, Mann-Whitney) while levels of erythrocyte TDP (an active TKT cofactor) in mid-trimester were significantly higher in GDM compared to controls (P = 0.04, Mann-Whitney). However, mid-gestational TKT activity - reflecting pentose phosphate pathway activity - did not differ between the two groups (P > 0.05, Mann-Whitney). Furthermore, we ascertained significant associations of postpartum TKT activity with SNPs SLC19A2 rs6656822 and SLC19A3 rs7567984 (P = 0.03 and P = 0.007, resp., Kruskal-Wallis). Our findings of increased thiamine delivery to the cells without concomitant increase of TKT activity in women with GDM therefore indicate possible pathogenic role of thiamine mishandling in GDM. Further studies are needed to determine its contribution to maternal and/or neonatal morbidity.

Department of Biochemistry Faculty of Medicine Masaryk University Brno Kamenice 5 625 00 Brno Czech Republic

Department of Pathophysiology Faculty of Medicine Masaryk University Brno UKB Kamenice 5 A18 625 00 Brno Czech Republic

Diabetes Centre Department of Internal Medicine Gastroenterology University Hospital Brno Jihlavská 20 625 00 Brno Czech Republic

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Diabetes. 2013 Aug;62(8):2689-98 PubMed

Ann Ist Super Sanita. 1997;33(3):375-8 PubMed

Diabetes Care. 2006 Jun;29(6):1420-32 PubMed

Diabetologia. 2007 Oct;50(10):2164-70 PubMed

J Pharm Biomed Anal. 2005 Apr 29;37(5):1025-9 PubMed

Curr Diab Rep. 2010 Jun;10(3):242-7 PubMed

J Am Coll Nutr. 2002 Feb;21(1):33-7 PubMed

Lancet. 2009 May 23;373(9677):1773-9 PubMed

Diabetes Obes Metab. 2013 Aug;15(8):677-89 PubMed

J Chromatogr. 1989 Jun 30;491(1):89-96 PubMed

Nat Genet. 1999 Jul;22(3):300-4 PubMed

Lancet. 2009 May 23;373(9677):1789-97 PubMed

Ann N Y Acad Sci. 1993 Mar 15;678:325-9 PubMed

World J Diabetes. 2014 Jun 15;5(3):288-95 PubMed

Am J Clin Nutr. 1975 Jan;28(1):59-65 PubMed

J Chromatogr A. 2000 Jun 9;881(1-2):267-84 PubMed

Ann Hum Genet. 2007 Sep;71(Pt 5):578-85 PubMed

Nephrol Dial Transplant. 2011 Apr;26(4):1229-36 PubMed

Diabetes. 2003 Aug;52(8):2110-20 PubMed

Amino Acids. 2012 Apr;42(4):1151-61 PubMed

Am J Clin Nutr. 1974 Nov;27(11):1221-4 PubMed

Diabetes Obes Metab. 2011 Jul;13(7):577-83 PubMed

Diabetes Care. 2011 Jul;34(7):1610-6 PubMed

J Perinatol. 2009 Jul;29(7):483-8 PubMed

Hypertens Pregnancy. 2008;27(4):374-86 PubMed

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016 Mar;160(1):70-5 PubMed

Diabetes. 2011 Oct;60(10):2523-32 PubMed

Am J Hum Genet. 2005 Jul;77(1):16-26 PubMed

Clin Chem. 2000 May;46(5):704-10 PubMed