Effect of salinity on the fatty acid and triacylglycerol composition of five haptophyte algae from the genera Coccolithophora, Isochrysis and Prymnesium determined by LC-MS/APCI
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
27298276
DOI
10.1016/j.phytochem.2016.06.001
PII: S0031-9422(16)30117-0
Knihovny.cz E-resources
- Keywords
- Coccolithophyceae, Haptophyte algae (genera Coccolithophora, Isochrysis and Prymnesium), Liquid chromatography-mass spectrometry, Octadecapentaenoic acid, Salinity, Stearidonic acid,
- MeSH
- Haptophyta chemistry genetics MeSH
- Fatty Acids analysis MeSH
- Molecular Structure MeSH
- Fatty Acids, Unsaturated chemistry MeSH
- Fatty Acids, Omega-3 chemistry MeSH
- Salinity MeSH
- Stereoisomerism MeSH
- Triglycerides analysis MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Germany MeSH
- Names of Substances
- Fatty Acids MeSH
- Fatty Acids, Unsaturated MeSH
- Fatty Acids, Omega-3 MeSH
- stearidonic acid MeSH Browser
- Triglycerides MeSH
Non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) with atmospheric pressure chemical ionization (APCI) was used for separation of triacylglycerols from five strains of haptophyte algae (genera Coccolithophora, Isochrysis, and Prymnesium). This study describes the separation and identification of C18 polyunsaturated triacylglycerols containing stearidonic and octadecapentaenoic fatty acids, including their regioisomers. Salinity affects the proportion of saturated and unsaturated fatty acids. The biosynthesis of C18 polyunsaturated triacylglycerols was found to be very stereospecific and to depend on the salinity of cultivation media, asymmetric regioisomers predominating at low salinity (sn-OpOpSt and/or PoStSt) and symmetric ones at high salinity (sn-OpStOp and or StPoSt).
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