Expression of nestin, CD133 and ABCG2 in relation to the clinical outcome in pediatric sarcomas
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
27314299
DOI
10.3233/cbm-160623
PII: CBM623
Knihovny.cz E-zdroje
- Klíčová slova
- Ewing sarcoma, Rhabdomyosarcoma, cancer stem cell markers, osteosarcoma, prognosis,
- MeSH
- ABC transportér z rodiny G, člen 2 genetika metabolismus MeSH
- antigen AC133 genetika metabolismus MeSH
- dítě MeSH
- dospělí MeSH
- exprese genu MeSH
- imunohistochemie MeSH
- Kaplanův-Meierův odhad MeSH
- kojenec MeSH
- lidé MeSH
- metastázy nádorů MeSH
- mladiství MeSH
- mladý dospělý MeSH
- nádorové biomarkery * MeSH
- nádorové kmenové buňky metabolismus MeSH
- nestin genetika metabolismus MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- prognóza MeSH
- sarkom diagnóza genetika metabolismus mortalita MeSH
- staging nádorů MeSH
- stupeň nádoru MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- ABC transportér z rodiny G, člen 2 MeSH
- antigen AC133 MeSH
- nádorové biomarkery * MeSH
- nestin MeSH
BACKGROUND: Nestin, CD133 and ABCG2 are recently discussed as putative markers, co-expression of which might determine a cancer stem cell (CSC) phenotype in sarcomas. OBJECTIVE: Our study is focused on immunohistochemical analysis of nestin, CD133 and ABCG2 expression in rhabdomyosarcoma, Ewing sarcoma and osteosarcoma. Furthermore, we also analyzed the possible correlation of nestin, CD133 and ABCG2 expression levels with the patient outcome to identify potential prognostic values of these three putative CSC markers in the same cohorts. METHODS: Using immunohistochemistry, expression of nestin, CD133 and ABCG2 was analyzed in 24 rhabdomyosarcoma, 22 Ewing sarcoma and 10 osteosarcoma tissue samples and expression levels of these markers were correlated with clinical outcome. RESULTS: High nestin levels indicate poor prognosis in patients with Ewing sarcoma (P = 0.001), and high CD133 expression is associated with shorter survival in rhabdomyosarcoma patients (P = 0.002). In contrast, no significant relationship was found between ABCG2 expression and the clinical outcome. CONCLUSIONS: Our analysis represents the first complex study of these three putative CSCs markers together in three different types of pediatric sarcomas and showed their possible prognostic values in these tumors.
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