In this research, a microbial endophytic strain obtained from the rhizosphere of the conifer Taxus baccata and designated as Streptomyces sp. AC35 (FJ001754.1 Streptomyces, GenBank) was investigated. High 16S rDNA gene sequence similarity suggests that this strain is closely related to S. odorifer. The major fatty acid profile of intracellular lipids was also carried out to further identify this strain. Atomic force microscopy and scanning acoustic microscopy were used to image our strain. Its major excreted substances were extracted, evaluated for antimicrobial activity, purified, and identified by ultraviolet-visible spectroscopy (UV-vis), liquid chromatography-mass spectrometry (LC-MS/MS) and nuclear magnetic resonance as the bioactive isoflavone aglycones-daidzein, glycitein and genistein. Batch cultivation, performed under different pH conditions, revealed enhanced production of antimycin components when the pH was stable at 7.0. Antimycins were detected by HPLC and identified by UV-vis and LC-MS/MS combined with the multiple reaction monitoring. Our results demonstrate that Streptomyces sp. AC35 might be used as a potential source of effective, pharmaceutically active compounds.

Department of Natural Drugs Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic

Institute of Biotechnology Faculty of Chemical and Food Technology Slovak University of Technology in Bratislava Bratislava Slovakia

Institute of Chemistry Center for Glycomics Slovak Academy of Sciences Bratislava Slovakia

See more in PubMed

Arch Microbiol. 2006 Jan;184(5):271-8 PubMed

J Basic Microbiol. 2010 Apr;50(2):135-42 PubMed

J Antibiot (Tokyo). 2005 Jan;58(1):1-26 PubMed

J Antibiot (Tokyo). 1970 Feb;23(2):81-90 PubMed

Biochim Biophys Acta. 1961 Oct 28;53:396-402 PubMed

Endocr Res. 2004 May;30(2):215-23 PubMed

J Bacteriol. 2007 Mar;189(6):2219-25 PubMed

Beilstein J Org Chem. 2013 Nov 19;9:2556-63 PubMed

Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2857-63 PubMed

J Chromatogr. 1989 Mar 3;464(2):453-8 PubMed

Biomed Pharmacother. 2007 Oct;61(9):591-5 PubMed

J Antibiot (Tokyo). 2005 Jan;58(1):74-8 PubMed

Appl Environ Microbiol. 2010 Jun;76(11):3702-14 PubMed

Bioimpacts. 2011;1(1):47-52 PubMed

J Antibiot (Tokyo). 2005 Jul;58(7):460-7 PubMed

Biosci Biotechnol Biochem. 2002 Dec;66(12):2626-31 PubMed

J Antibiot (Tokyo). 2011 Oct;64(10):661-665 PubMed

Brief Bioinform. 2004 Jun;5(2):150-63 PubMed

J Gen Appl Microbiol. 2003 Jun;49(3):141-54 PubMed

Anal Biochem. 1976 May 7;72:248-54 PubMed

Mar Drugs. 2014 Jun 02;12(6):3323-51 PubMed

Int J Syst Bacteriol. 1997 Jan;47(1):202-6 PubMed

J Antibiot (Tokyo). 2007 Jan;60(1):65-72 PubMed

Microbes Infect. 2004 Jan;6(1):86-92 PubMed