Detection of selected periodontal bacteria in preschool children affected by early childhood caries
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
27485551
DOI
10.1007/s12223-016-0468-5
PII: 10.1007/s12223-016-0468-5
Knihovny.cz E-resources
- MeSH
- Bacteria classification isolation & purification MeSH
- Bacterial Infections complications microbiology MeSH
- Bacteriological Techniques MeSH
- Molecular Diagnostic Techniques MeSH
- Humans MeSH
- Periodontitis microbiology MeSH
- Child, Preschool MeSH
- Dental Caries complications microbiology MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
The aim of this study was to compare the detection frequency of periodontal bacteria in dental plaque in children with early childhood caries (ECC) with and without gingival inflammation. A convenience sample of 25 preschool children (mean age 3.61 years, SD 1.42) was recruited. Dental plaque was taken from periodontal areas with and without visible signs of inflammation and processed using the StomaGene® (Protean s.r.o. Czech Republic) and ParoCheck® 20 (Greiner Bio-one GmbH, Germany) detection kits. The two sample t tests between percents for differences between inflammatory and healthy sites and kappa statistics for the agreement of both systems were used. At the inflammatory sites, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were significantly more frequently detected by StomaGene® while Fusobacterium nucleatum, A. actinomycetemcomitans, Tanarella forsythia and Prevotella intermedia were significantly more frequently identified by ParoCheck® test. The agreement between the two detection systems was substantial for A. actinomycetemcomitans and F. nucleatum in the samples collected from inflamed sites and only for F. nucleatum from clinically healthy sites. Therefore, we recommend that the same system should be used when the same patient is examined repeatedly.
Department of Pathophysiology Faculty of Medicine Masaryk University Brno Czech Republic
School of Dental Medicine the 1st Faculty of Medicine Charles University Prague Czech Republic
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