Localized Movement and Levels of 53BP1 Protein Are Changed by γ-irradiation in PML Deficient Cells
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27526954
DOI
10.1002/jcb.25551
Knihovny.cz E-resources
- Keywords
- 53BP1 PROTEIN, DNA REPAIR, HISTONE MODIFICATIONS, MICROSCOPY, PHOTOBLEACHING, PML BODIES,
- MeSH
- Tumor Suppressor p53-Binding Protein 1 metabolism MeSH
- Leukemia, Promyelocytic, Acute metabolism pathology radiotherapy MeSH
- Fluorescent Antibody Technique MeSH
- Intranuclear Inclusion Bodies metabolism pathology radiation effects MeSH
- Microscopy, Confocal MeSH
- Humans MeSH
- Tumor Cells, Cultured MeSH
- DNA Repair physiology radiation effects MeSH
- DNA Damage physiology radiation effects MeSH
- Dose-Response Relationship, Radiation MeSH
- Blotting, Western MeSH
- Gamma Rays adverse effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Tumor Suppressor p53-Binding Protein 1 MeSH
- TP53BP1 protein, human MeSH Browser
We studied epigenetics, distribution pattern, kinetics, and diffusion of proteins recruited to spontaneous and γ-radiation-induced DNA lesions. We showed that PML deficiency leads to an increased number of DNA lesions, which was accompanied by changes in histone signature. In PML wt cells, we observed two mobile fractions of 53BP1 protein with distinct diffusion in spontaneous lesions. These protein fractions were not detected in PML-deficient cells, characterized by slow-diffusion of 53BP1. Single particle tracking analysis revealed limited local motion of 53BP1 foci in PML double null cells and local motion 53BP1 foci was even more reduced after γ-irradiation. However, radiation did not change co-localization between 53BP1 nuclear bodies and interchromatin granule-associated zones (IGAZs), nuclear speckles, or chromocenters. This newly observed interaction pattern imply that 53BP1 protein could be a part of not only DNA repair, but also process mediated via components accumulated in IGAZs, nuclear speckles, or paraspeckles. Together, PML deficiency affected local motion of 53BP1 nuclear bodies and changed composition and a number of irradiation-induced foci. J. Cell. Biochem. 117: 2583-2596, 2016. © 2016 Wiley Periodicals, Inc.
Department of Oral Biology University of Oslo Oslo Norway
Faculty of Informatics Masaryk University Botanická 68a Brno 602 00 Czech Republic
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