High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Arabidopsis, Cytokinins, Fluorescence-activated cell sorting (FACS), Liquid chromatography–mass spectrometry (LC-MS), Protoplasts, Roots, Solid-phase extraction (SPE),
- MeSH
- Arabidopsis metabolism MeSH
- Cytokinins metabolism MeSH
- Gene Regulatory Networks physiology MeSH
- Plant Roots metabolism MeSH
- Arabidopsis Proteins metabolism MeSH
- Flow Cytometry methods MeSH
- Plant Growth Regulators metabolism MeSH
- Tandem Mass Spectrometry methods MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytokinins MeSH
- Arabidopsis Proteins MeSH
- Plant Growth Regulators MeSH
We describe a method combining fluorescence-activated cell sorting (FACS) with one-step miniaturized isolation and accurate quantification of cytokinins (CKs) using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to measure these phytohormones in specific cell types of Arabidopsis thaliana roots. The methodology provides information of unprecedented resolution about spatial distributions of CKs, and thus should facilitate attempts to elucidate regulatory networks involved in root developmental processes.
References provided by Crossref.org
Cell-surface receptors enable perception of extracellular cytokinins
