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Isolation and characterization of a novel glycosyl hydrolase family 74 (GH74) cellulase from the black goat rumen metagenomic library

Song, Yun-Hee
Author Song, Yun-Hee Department of Food Science and Technology, Chung-Ang University, Ansung, 456-756, South Korea
Lee, Kyung-Tai
Author Lee, Kyung-Tai Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Wanju, 565-851, South Korea
Baek, Jin-Young
Author Baek, Jin-Young Department of Food Science and Technology, Chung-Ang University, Ansung, 456-756, South Korea
Kim, Min-Ju
Author Kim, Min-Ju Department of Food Science and Technology, Chung-Ang University, Ansung, 456-756, South Korea
Kwon, Mi-Ra
Author Kwon, Mi-Ra Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Wanju, 565-851, South Korea
Kim, Young-Joo
Author Kim, Young-Joo Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Wanju, 565-851, South Korea
Park, Mi-Rim
Author Park, Mi-Rim Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Wanju, 565-851, South Korea
Ko, Haesu
Author Ko, Haesu Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Wanju, 565-851, South Korea
Lee, Jin-Sung
Author Lee, Jin-Sung Department of Biological Sciences, Kyonggi University, Suwon, 442-760, South Korea
Kim, Keun-Sung
Author Kim, Keun-Sung Department of Food Science and Technology, Chung-Ang University, Ansung, 456-756, South Korea. keunsung@cau.ac.kr

Folia microbiologica. 2017 May ; 62 (3) : 175-181. [epub] 20161119

Folia Microbiol (Praha)
ISSN 1874-9356 | 0015-5632
Source

Language English Country United States Media print-electronic

Document type Journal Article

Persistent link https://www.medvik.cz/link/pmid27866354

PubMed 27866354
DOI 10.1007/s12223-016-0486-3
PII: 10.1007/s12223-016-0486-3
Knihovny.cz E-resources

MeSH
Rumen microbiology MeSH
Cellulase chemistry genetics isolation & purification MeSH
Escherichia coli genetics metabolism MeSH
Gene Expression MeSH
Fibrobacter enzymology genetics MeSH
Genetic Testing MeSH
Gene Library MeSH
Cloning, Molecular MeSH
Hydrogen-Ion Concentration MeSH
Goats microbiology MeSH
Metagenome * MeSH
Metagenomics MeSH
Molecular Weight MeSH
Recombinant Proteins genetics isolation & purification metabolism MeSH
Sequence Homology MeSH
Enzyme Stability MeSH
Substrate Specificity MeSH
Temperature MeSH
Animals MeSH
Check Tag
Animals MeSH
Publication type
Journal Article MeSH
Names of Substances
Cellulase MeSH
Recombinant Proteins MeSH

This study aimed to isolate and characterize a novel cellulolytic enzyme from black goat rumen by using a culture-independent approach. A metagenomic fosmid library was constructed from black goat rumen contents and screened for a novel cellulase. The KG37 gene encoding a protein of 858 amino acid residues (92.7 kDa) was isolated. The deduced protein contained a glycosyl hydrolase family 74 (GH74) domain and showed 77% sequence identity to two endo-1,4-β-glucanases from Fibrobacter succinogenes. The novel GH74 cellulase gene was overexpressed in Escherichia coli, and its protein product was functionally characterized. The recombinant GH74 cellulase showed a broad substrate spectrum. The enzyme exhibited its optimum activity at pH 5.0 and temperature range of 20-50 °C. The enzyme was thermally stable at pH 5.0 and at a temperature of 20-40 °C. The novel GH74 cellulase can be practically exploited to convert lignocellulosic biomass to value-added products in various industrial applications in future.

Animal Genomics and Bioinformatics Division National Institute of Animal Science Rural Development Administration Wanju 565 851 South Korea

Department of Biological Sciences Kyonggi University Suwon 442 760 South Korea

Department of Food Science and Technology Chung Ang University Ansung 456 756 South Korea

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