A recently presented new type of "multilayered" organic-inorganic hybrid silica particle packed column YMC-Triart C18 (50 mm × 4.6 mm, 5 μm) was used for the development of a sequential injection chromatography method for determination of five azo dyes (Sudan I, Sudan II, Sudan III, Sudan orange G, and para red) in selected food seasonings. The use of a novel sorbent brings attractive features, reduced backpressure, and broader chemical stability together with high separation performance, which are discussed and compared with that of three types of columns typically used in medium-pressure flow chromatography techniques (classic monolithic, narrow monolithic, and core-shell particle columns). The separation was performed in gradient elution mode created by the zone mixing of two mobile phases (acetonitrile/water 90:10, 1.5 mL + acetonitrile/water 100:0, 2.3 mL) at a flow rate of 0.60 mL/min and time of analysis <9.5 min. The spectrophotometric detection wavelengths were set to 400, 480, and 500 nm. The high performance of the developed method with multilayered particle column was well documented and the results indicate a broad capability of sequential injection chromatography.

Department of Analytical Chemistry Faculty of Pharmacy in Hradec Králové Charles University Hradec Králové Czech Republic

Department of Chemical Sciences Faculty of Pharmacy University of Porto Porto Portugal

INQUISUR Department of Chemistry Universidad Nacional del Sur Bahía Blanca Argentina

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A simple method to quantify azo dyes in spices based on flow injection chromatography combined with chemometric tools