Toward ideal separation by size-exclusion chromatography
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28139225
DOI
10.1016/j.chroma.2017.01.038
PII: S0021-9673(17)30106-1
Knihovny.cz E-zdroje
- Klíčová slova
- Band broadening function, Diffusion, Excess kurtosis, Separation mechanism, Size-exclusion chromatography, Skew,
- MeSH
- chemické techniky analytické metody MeSH
- difuze MeSH
- gelová chromatografie * MeSH
- molekulová hmotnost MeSH
- polymery chemie izolace a purifikace MeSH
- polystyreny chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- polymery MeSH
- polystyreny MeSH
In analyses of polystyrene standards by size-exclusion chromatography for polymers of molecular weight about thirty thousand and higher, the separation improved with decreasing flow-rate is in accord with Giddings observation of transversal diffusion as a factor decreasing the broadening of the band broadening function. The variance of the elution curves is larger than it corresponds to dispersity M¯w/M¯n and the shape of the elution curves depends on the experimental conditions which suggests that it reflects properties of the band broadening function. The skew and the excess kurtosis of the elution curves increase near the exclusion limit in accord with theoretical prediction for the band broadening function.
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