Two-photon laser-scanning microscopy for single and repetitive imaging of dorsal and lateral spinal white matter in vivo
Language English Country Czech Republic Media print-electronic
Document type Journal Article
PubMed
28248542
DOI
10.33549/physiolres.933461
PII: 933461
Knihovny.cz E-resources
- MeSH
- White Matter cytology diagnostic imaging MeSH
- Microscopy, Confocal methods MeSH
- Laser Scanning Cytometry methods MeSH
- Spinal Cord cytology diagnostic imaging MeSH
- Mice, Inbred C57BL MeSH
- Mice, Transgenic MeSH
- Mice MeSH
- Organ Culture Techniques MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
We developed appropriate surgical procedures for single and repetitive multi-photon imaging of spinal cord in vivo. By intravenous anesthesia, artificial ventilation and laminectomy, acute experiments were performed in the dorsal and lateral white matter. By volatile anesthesia and minimal-invasive surgery, chronic repetitive imaging up to 8 months were performed in the dorsal column through the window between two adjacent spines. Transgenic mouse technology enabled simultaneous imaging of labeled axons, astrocytes and microglia. Repetitive imaging showed positional shifts of microglia over time. These techniques serve for investigations of cellular dynamics and cell-cell interactions in intact and pathologically changed spinal tissue.
References provided by Crossref.org
