Use of Translation Blocking Morpholinos for Gene Knockdown in Giardia lamblia
Language English Country United States Media print
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
R01 AI110708
NIAID NIH HHS - United States
- Keywords
- Giardia, Integration, Knockdown, Morpholino, Quantitative Western blotting,
- MeSH
- Electroporation MeSH
- Gene Expression MeSH
- Genetic Vectors genetics MeSH
- Gene Knockdown Techniques * MeSH
- Giardia lamblia genetics immunology metabolism MeSH
- Cloning, Molecular MeSH
- Morpholinos administration & dosage chemistry genetics MeSH
- Gene Order MeSH
- Protein Biosynthesis genetics MeSH
- Protozoan Proteins genetics metabolism MeSH
- Recombinant Fusion Proteins MeSH
- Base Sequence MeSH
- Transformation, Genetic MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- Names of Substances
- Morpholinos MeSH
- Protozoan Proteins MeSH
- Recombinant Fusion Proteins MeSH
Giardia lamblia, a major parasite, is an emerging model organism due to its compact genomic arrangement and composition. The most popular reverse genetic technique, RNAi, is ineffective in Giardia. In contrast, protein depletion by translation blocking morpholinos is suitable for most gene targets and provides up to 80% depletion of the target protein. The method is fast, reliable, and specific. After antisense morpholino oligomer delivery into Giardia trophozoites by electroporation, the cells can be used for many subsequent analyses 8-48 h after treatment. In this chapter, suitable gene tags, plasmids, and techniques necessary for proper morpholino targeting are described.
References provided by Crossref.org
The Giardia ventrolateral flange is a lamellar membrane protrusion that supports attachment
14-3-3 Regulates Actin Filament Formation in the Deep-Branching Eukaryote Giardia lamblia
