Možnosti implementace sekvenace celého genomu (WGS) Neisseria meningitidis do molekulární surveillance invazivního meningokokového onemocnění v České republice
[The possibilities for the implementation of whole genome sequencing (WGS) of Neisseria meningitidis in molecular surveillance of invasive meningococcal disease in the Czech Republic]
Jazyk čeština Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
28374598
PII: 60672
- Klíčová slova
- whole genome sequencing - WGS - Neisseria meningitides - molecular surveillance.,
- MeSH
- epidemiologické monitorování MeSH
- incidence MeSH
- lidé MeSH
- meningokokové infekce * epidemiologie mikrobiologie MeSH
- Neisseria meningitidis * genetika MeSH
- sekvenování celého genomu * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
The implementation of the surveillance of invasive meningococcal disease is recommended worldwide. The Whole Genome Sequencing (WGS) method increasingly comes to the fore, which provides the possibilities for further detailed characterization of Neisseria meningitidis and makes it possible to integrate all conventional sequencing approaches into one method. Six N. meningitidis isolates from 2013 and 2015, characterized previously by Sanger amplicon sequencing, were selected to be studied by the novel WGS method. WGS data analysis has confirmed the accuracy of this method in determining epidemiological markers. The aim of this communication is to point out the possibility for the implementation of WGS into molecular surveillance of invasive meningococcal disease in the Czech Republic. The National Reference Laboratory for Meningococcal Infections (NRL/MEN) will continue to use WGS for molecular characterization of selected isolates of N. meningitidis and for the improvement of molecular surveillance of invasive meningococcal disease in the country.
