Changes of Cerebrospinal Fluid Peptides due to Tauopathy
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
28453489
DOI
10.3233/jad-170110
PII: JAD170110
Knihovny.cz E-resources
- Keywords
- Cerebrospinal fluid, LC-MALDI MS, peptidomics, rat model, tauopathy,
- MeSH
- Chromatography, Liquid MeSH
- Rats MeSH
- Humans MeSH
- Disease Models, Animal MeSH
- Peptides cerebrospinal fluid MeSH
- Rats, Transgenic MeSH
- tau Proteins genetics MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Tauopathies cerebrospinal fluid genetics MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Peptides MeSH
- tau Proteins MeSH
Alzheimer's disease (AD) and progressive supranuclear palsy are two common neurodegenerative tauopathies, and the most common cause of progressive brain dementia in elderly affecting more than 35 million people. The tauopathies are characterized by abnormal deposition of microtubule associated protein tau into intracellular neurofibrillary tangles composed mainly of the hyperphosphorylated form of the protein. The diagnosis of tauopathies is based on the presence of clinical features and pathological changes. Over the last decade, there has been an intensive search for novel biochemical markers for clinical diagnosis of AD and other tauopathies. In the present study, we used transgenic rat model for tauopathy expressing human truncated tau protein (aa 151-391/4R) to analyze the cerebrospinal fluid (CSF) peptidome using liquid chromatography - matrix assisted laser desorption/ionization mass spectrometry (LC-MALDI TOF/TOF). From 345 peptides, we identified a total of 175 proteins. Among them, 17 proteins were significantly altered in the CSF of transgenic rats. The following proteins were elevated in the CSF of transgenic rats when compared to the control animals: neurofilament light and medium chain, apolipoprotein E, gamma-synuclein, chromogranin A, reticulon-4, secretogranin-2, calsyntein-1 and -3, endothelin-3, neuroendocrine protein B72A, alpha-1-macroglobulin, and augurin. Interestingly most of the identified proteins were previously linked to AD and other tauopathies, indicating the significance of transgenic animals in biomarker validation.
AXON Neuroscience R and D Bratislava Slovak Republic
Institute of Chemistry Slovak Academy of Sciences Bratislava Slovak Republic
Institute of Mathematics and Statistics Faculty of Science Masaryk University Brno Czech Republic
Institute of Neuroimmunology Slovak Academy of Sciences Bratislava Slovak Republic
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