3D Coculture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial-Stromal Interactions During Mammary Branching Morphogenesis
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- 3D culture, Branching morphogenesis, Collagen, Extracellular matrix, Fibroblasts, Mammary gland, Matrigel, Organoids, Paracrine signaling, Spheroid,
- MeSH
- Cell Differentiation MeSH
- Spheroids, Cellular cytology MeSH
- Stromal Cells cytology MeSH
- Epithelial Cells cytology MeSH
- Fibroblasts cytology MeSH
- Coculture Techniques methods MeSH
- Cells, Cultured MeSH
- Mammary Glands, Animal cytology MeSH
- Mice MeSH
- Organoids cytology MeSH
- Paracrine Communication MeSH
- Cell Proliferation MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Mammary gland is composed of branched epithelial structure embedded within a complex stroma formed by several stromal cell types, including fibroblasts, and extracellular matrix (ECM). Development of mammary gland is tightly regulated by bidirectional epithelial-stromal interactions that include paracrine signaling, ECM remodeling and mechanosensing. Importantly, these interactions play crucial role in mammary gland homeostasis and when deregulated they contribute to tumorigenesis. Therefore, understanding the mechanisms underlying epithelial-stromal interactions is critical for elucidating regulation of normal mammary gland development and homeostasis and revealing novel strategies for breast cancer therapy. To this end, several three-dimensional (3D) cell culture models have been developed to study these interactions in vitro. In this chapter, a novel 3D organoid-fibrosphere coculture model of mammary gland is described with the capacity for studying not only the qualitative and quantitative aspects of interactions between mammary fibroblasts and epithelial organoids but also their radius and directionality.
References provided by Crossref.org
Fibroblast-induced mammary epithelial branching depends on fibroblast contractility
An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast
Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks
Primary Mammary Organoid Model of Lactation and Involution
