The polymorphic insertion of the luteinizing hormone receptor "insLQ" show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
28684292
DOI
10.1016/j.mce.2017.07.002
PII: S0303-7207(17)30356-8
Knihovny.cz E-zdroje
- Klíčová slova
- Follicle fluid, Human small antral follicles, LHR, rs4539842,
- MeSH
- dospělí MeSH
- folikulární buňky metabolismus MeSH
- folikulární tekutina metabolismus MeSH
- genotyp MeSH
- hormony metabolismus MeSH
- inzerční mutageneze * MeSH
- lidé MeSH
- messenger RNA genetika metabolismus MeSH
- mladiství MeSH
- mladý dospělý MeSH
- ovariální folikul metabolismus MeSH
- polymorfismus genetický * MeSH
- receptory LH genetika MeSH
- regulace genové exprese * MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- hormony MeSH
- messenger RNA MeSH
- receptory LH MeSH
The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by qPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Müllerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ/insLQ compared to -/insLQ and the -/- genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in -/insLQ compared to the -/- genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.
Department of Biomedicine University of Aarhus Aarhus Denmark
Laboratory of Reproductive Biology Copenhagen University Hospital Rigshospitalet Copenhagen Denmark
The Fertility Clinic Aarhus University Hospital Aarhus Denmark
The Fertility Clinic Copenhagen University Hospital Rigshospitalet Copenhagen Denmark
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