Aporocotylid blood flukes conspecific with Aporocotyle margolisi Smith, 1967 were collected from the bulbus arteriosus of the North Pacific hake Merluccius productus (Ayres). This study revisits the morphology of A. margolisi, including drawings, measurements and scanning electron microscopy images, and provides for the first time molecular data for the large subunit of the ribosomal RNA (28S rDNA) and the mitochondrial cytochrome c oxidase subunit 1 (cox1) genes for this species. A 28S rDNA phylogenetic study of A. margolisi, and all available Aporocotyle spp., was also performed. The distribution range of A. margolisi is extended to the Pacific coast of the USA. We provide a morphological comparison of Aporocotyle spp. from the Pacific coast in North America as well as other Aporocotyle spp. infecting hake. Comparisons with the original description revealed that the new specimens of A. margolisi were considerably larger with respect to all morphological features, except for shorter spines. Molecular results showed a close relationship between A. margolisi and A. argentinensis Smith, 1969 from the Argentine hake Merluccius hubbsi Marini. The phylogenetic relationships of Aporocotyle spp. point to a possible co-speciation of hakes species and these blood fluke parasites.

Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos Almirante Storni Universidad Nacional del Comahue Güemes 1030 8520 San Antonio Oeste Río Negro Argentina

Department of Microbiology Oregon State University Corvallis OR 97331 USA

Institute of Parasitology Biology Centre of the Czech Academy of Sciences 37005 České Budějovice Czech Republic

Laboratorio de Helmintología Instituto de Biología Universidad Nacional Autónoma de México Avenida Universidad 3000 Ciudad Universitaria 04510 Mexico City Mexico

Newport Research Station Northwest Fisheries Science Center National Marine Fisheries Service 2030 S E Marine Science Dr Newport OR 97365 USA

Posgrado en Ciencias Biológicas Universidad Nacional Autónoma de México Ciudad Universitaria 04510 Mexico City Mexico

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