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Tyndall JD, Sandilya R. GPCR agonists and antagonists in the clinic. Med. Chem. 2005;1:405–421. doi: 10.2174/1573406054368675.
PubMed
DOI
Marinissen MJ, Gutkind JS. G-protein-coupled receptors and signaling networks: emerging paradigms. Trends. Pharmacol. Sci. 2001;22:368–376. doi: 10.1016/S0165-6147(00)01678-3.
PubMed
DOI
Alexander SP, et al. The concise guide to PHARMACOLOGY 2013/14: overview. Br. J. Pharmacol. 2013;170:1449–1458. doi: 10.1111/bph.12444.
PubMed
DOI
PMC
Whorton MR, et al. A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. Proc. Natl Acad. Sci. USA. 2007;104:7682–7687. doi: 10.1073/pnas.0611448104.
PubMed
DOI
PMC
Milligan G. G protein-coupled receptor dimerization: function and ligand pharmacology. Mol. Pharmacol. 2004;66:1–7. doi: 10.1124/mol.104.000497..
PubMed
DOI
Ferre S, et al. G protein-coupled receptor oligomerization revisited: functional and pharmacological perspectives. Pharmacol. Rev. 2014;66:413–434. doi: 10.1124/pr.113.008052.
PubMed
DOI
PMC
Bouvier M, Hebert TE. CrossTalk proposal: weighing the evidence for Class A GPCR dimers, the evidence favours dimers. J. Physiol. 2014;592:2439–2441. doi: 10.1113/jphysiol.2014.272252.
PubMed
DOI
PMC
Schulte G. International union of basic and clinical pharmacology. LXXX. The class Frizzled receptors. Pharmacol. Rev. 2010;62:632–667. doi: 10.1124/pr.110.002931.
PubMed
DOI
Koval A, Katanaev VL. Platforms for high-throughput screening of Wnt/Frizzled antagonists. Drug Discov. Today. 2012;17:1316–1322. doi: 10.1016/j.drudis.2012.07.007.
PubMed
DOI
Dijksterhuis JP, Petersen J, Schulte G. WNT/Frizzled signalling: receptor-ligand selectivity with focus on FZD-G protein signalling and its physiological relevance: IUPHAR review 3. Br. J. Pharmacol. 2014;171:1195–1209. doi: 10.1111/bph.12364.
PubMed
DOI
PMC
Janda CY, Waghray D, Levin AM, Thomas C, Garcia KC. Structural basis of Wnt recognition by Frizzled. Science. 2012;337:59–64. doi: 10.1126/science.1222879.
PubMed
DOI
PMC
Schulte G. Frizzleds and WNT/beta-catenin signaling - the black box of ligand-receptor selectivity, complex stoichiometry and activation kinetics. Eur. J. Pharmacol. 2015;763:191–195. doi: 10.1016/j.ejphar.2015.05.031.
PubMed
DOI
van Amerongen R, Mikels A, Nusse R. Alternative wnt signaling is initiated by distinct receptors. Sci. Signal. 2008;1:re9.
PubMed
Kaykas A, et al. Mutant Frizzled 4 associated with vitreoretinopathy traps wild-type Frizzled in the endoplasmic reticulum by oligomerization. Nat. Cell. Biol. 2004;6:52–58. doi: 10.1038/ncb1081.
PubMed
DOI
Carron C, et al. Frizzled receptor dimerization is sufficient to activate the Wnt/beta-catenin pathway. J. Cell. Sci. 2003;116:2541–2550. doi: 10.1242/jcs.00451.
PubMed
DOI
Kilander MB, et al. Disheveled regulates precoupling of heterotrimeric G proteins to Frizzled 6. FASEB J. 2014;28:2293–2305. doi: 10.1096/fj.13-246363.
PubMed
DOI
Tauriello DV, et al. Wnt/beta-catenin signaling requires interaction of the Dishevelled DEP domain and C terminus with a discontinuous motif in Frizzled. Proc. Natl Acad. Sci. USA. 2012;109:E812–E820. doi: 10.1073/pnas.1114802109.
PubMed
DOI
PMC
Umbhauer M, et al. The C-terminal cytoplasmic Lys-thr-X-X-X-Trp motif in Frizzled receptors mediates Wnt/beta-catenin signalling. EMBO. J. 2000;19:4944–4954. doi: 10.1093/emboj/19.18.4944.
PubMed
DOI
PMC
Dorsch S, Klotz KN, Engelhardt S, Lohse MJ, Bunemann M. Analysis of receptor oligomerization by FRAP microscopy. Nat. Methods. 2009;6:225–230. doi: 10.1038/nmeth.1304.
PubMed
DOI
Lambert NA. Uncoupling diffusion and binding in FRAP experiments. Nat Methods. 2009;6:183–184. doi: 10.1038/nmeth0309-183a.
PubMed
DOI
PMC
Qin K, Sethi PR, Lambert NA. Abundance and stability of complexes containing inactive G protein-coupled receptors and G proteins. FASEB J. 2008;22:2920–2927. doi: 10.1096/fj.08-105775.
PubMed
DOI
PMC
Thews E, et al. Cross talk free fluorescence cross correlation spectroscopy in live cells. Biophys. J. 2005;89:2069–2076. doi: 10.1529/biophysj.104.057919.
PubMed
DOI
PMC
Wang C, et al. Structure of the human smoothened receptor bound to an antitumour agent. Nature. 2013;497:338–343. doi: 10.1038/nature12167.
PubMed
DOI
PMC
Proffitt KD, et al. Pharmacological inhibition of the Wnt acyltransferase PORCN prevents growth of WNT-driven mammary cancer. Cancer Res. 2013;73:502–507. doi: 10.1158/0008-5472.CAN-12-2258.
PubMed
DOI
Frojmark AS, et al. Mutations in Frizzled 6 cause isolated autosomal-recessive nail dysplasia. Am. J. Hum. Genet. 2011;88:852–860. doi: 10.1016/j.ajhg.2011.05.013.
PubMed
DOI
PMC
Kilander MB, Dahlstrom J, Schulte G. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal. 2014;26:1943–1949. doi: 10.1016/j.cellsig.2014.05.012.
PubMed
DOI
Shenoy SK, Lefkowitz RJ. Trafficking patterns of beta-arrestin and G protein-coupled receptors determined by the kinetics of beta-arrestin deubiquitination. J. Biol. Chem. 2003;278:14498–14506. doi: 10.1074/jbc.M209626200.
PubMed
DOI
Piga R, van Dartel D, Bunschoten A, van der Stelt I, Keijer J. Role of Frizzled6 in the molecular mechanism of beta-carotene action in the lung. Toxicology. 2014;320:67–73. doi: 10.1016/j.tox.2014.03.002.
PubMed
DOI
Tokuhara M, Hirai M, Atomi Y, Terada M, Katoh M. Molecular cloning of human Frizzled-6. Biochem. Biophys. Res. Commun. 1998;243:622–627. doi: 10.1006/bbrc.1998.8143.
PubMed
DOI
Uhlen M, et al. A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol. Cell. Proteomics. 2005;4:1920–1932. doi: 10.1074/mcp.M500279-MCP200.
PubMed
DOI
Wikenheiser KA, et al. Production of immortalized distal respiratory epithelial cell lines from surfactant protein C/simian virus 40 large tumor antigen transgenic mice. Proc. Natl Acad. Sci. USA. 1993;90:11029–11033. doi: 10.1073/pnas.90.23.11029.
PubMed
DOI
PMC
Han Y, Moreira IS, Urizar E, Weinstein H, Javitch JA. Allosteric communication between protomers of dopamine class A GPCR dimers modulates activation. Nat. Chem. Biol. 2009;5:688–695. doi: 10.1038/nchembio.199.
PubMed
DOI
PMC
Milligan G. The role of dimerisation in the cellular trafficking of G-protein-coupled receptors. Curr. Opin. Pharmacol. 2010;10:23–29. doi: 10.1016/j.coph.2009.09.010.
PubMed
DOI
Pin JP, Galvez T, Prezeau L. Evolution, structure, and activation mechanism of family 3/C G-protein-coupled receptors. Pharmacol. Ther. 2003;98:325–354. doi: 10.1016/S0163-7258(03)00038-X.
PubMed
DOI
Xue L, et al. Major ligand-induced rearrangement of the heptahelical domain interface in a GPCR dimer. Nat. Chem. Biol. 2015;11:134–140. doi: 10.1038/nchembio.1711.
PubMed
DOI
Zhao Y, Tong C, Jiang J. Hedgehog regulates smoothened activity by inducing a conformational switch. Nature. 2007;450:252–258. doi: 10.1038/nature06225.
PubMed
DOI
Dann CE, et al. Insights into Wnt binding and signalling from the structures of two Frizzled cysteine-rich domains. Nature. 2001;412:86–90. doi: 10.1038/35083601.
PubMed
DOI
Calebiro D, et al. Single-molecule analysis of fluorescently labeled G-protein-coupled receptors reveals complexes with distinct dynamics and organization. Proc. Natl Acad. Sci. USA. 2013;110:743–748. doi: 10.1073/pnas.1205798110.
PubMed
DOI
PMC
Kasai RS, Kusumi A. Single-molecule imaging revealed dynamic GPCR dimerization. Curr. Opin. Cell Biol. 2014;27:78–86. doi: 10.1016/j.ceb.2013.11.008.
PubMed
DOI
Hern JA, et al. Formation and dissociation of M1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules. Proc. Natl Acad. Sci. USA. 2010;107:2693–2698. doi: 10.1073/pnas.0907915107.
PubMed
DOI
PMC
Ward RJ, Pediani JD, Godin AG, Milligan G. Regulation of oligomeric organization of the serotonin 5-Hydroxytryptamine 2C (5-HT2C) receptor observed by spatial intensity distribution analysis. J. Biol. Chem. 2015;290:12844–12857. doi: 10.1074/jbc.M115.644724.
PubMed
DOI
PMC
Furness SG, et al. Ligand-dependent modulation of G protein conformation alters drug efficacy. Cell. 2016;167:739–749 e11. doi: 10.1016/j.cell.2016.09.021.
PubMed
DOI
Vafabakhsh R, Levitz J, Isacoff EY. Conformational dynamics of a class C G-protein-coupled receptor. Nature. 2015;524:497–501. doi: 10.1038/nature14679.
PubMed
DOI
PMC
Wu B, et al. Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists. Science. 2010;330:1066–1071. doi: 10.1126/science.1194396.
PubMed
DOI
PMC
Rasmussen SG, et al. Structure of a nanobody-stabilized active state of the beta(2) adrenoceptor. Nature. 2011;469:175–180. doi: 10.1038/nature09648.
PubMed
DOI
PMC
Rasmussen SG, et al. Crystal structure of the b2 adrenergic receptor-Gs protein complex. Nature. 2011;477:549–555. doi: 10.1038/nature10361.
PubMed
DOI
PMC
Chang L, Karin M. Mammalian MAP kinase signalling cascades. Nature. 2001;410:37–40. doi: 10.1038/35065000.
PubMed
DOI
De Lean A, Stadel JM, Lefkowitz RJ. A ternary complex model explains the agonist-specific binding properties of the adenylate cyclase-coupled beta-adrenergic receptor. J. Biol. Chem. 1980;255:7108–7117.
PubMed
Cordomi A, Navarro G, Aymerich MS, Franco R. Structures for G-protein-coupled receptor tetramers in complex with G proteins. Trends Biochem. Sci. 2015;40:548–551. doi: 10.1016/j.tibs.2015.07.007.
PubMed
DOI
Szczepek M, et al. Crystal structure of a common GPCR-binding interface for G protein and arrestin. Nat. Commun. 2014;5:4801. doi: 10.1038/ncomms5801.
PubMed
DOI
PMC
Phair RD, Gorski SA, Misteli T. Measurement of dynamic protein binding to chromatin in vivo, using photobleaching microscopy. Methods Enzymol. 2004;375:393–414. doi: 10.1016/S0076-6879(03)75025-3.
PubMed
DOI
Stromqvist J, et al. A modified FCCS procedure applied to Ly49A-MHC class I cis-interaction studies in cell membranes. Biophys. J. 2011;101:1257–1269. doi: 10.1016/j.bpj.2011.06.057.
PubMed
DOI
PMC
Bacia K, Schwille P. Practical guidelines for dual-color fluorescence cross-correlation spectroscopy. Nat. Protoc. 2007;2:2842–2856. doi: 10.1038/nprot.2007.410.
PubMed
DOI
Bacia K, Petrasek Z, Schwille P. Correcting for spectral cross-talk in dual-color fluorescence cross-correlation spectroscopy. Chemphyschem. 2012;13:1221–1231. doi: 10.1002/cphc.201100801.
PubMed
DOI
PMC
Wang C, et al. Structural basis for Smoothened receptor modulation and chemoresistance to anticancer drugs. Nat. Commun. 2014;5:4355.
PubMed
PMC
Larkin MA, et al. Clustal W and Clustal X version 2.0. Bioinformatics. 2007;23:2947–2948. doi: 10.1093/bioinformatics/btm404.
PubMed
DOI
Sali A, Blundell TL. Comparative protein modelling by satisfaction of spatial restraints. J. Mol. Biol. 1993;234:779–815. doi: 10.1006/jmbi.1993.1626.
PubMed
DOI
Berendsen HJ, van der Spoel D, van Drunen R. GROMACS: a message-passing parallel molecular dynamics implementation. Comput. Phys. Commun. 1995;91:43–56. doi: 10.1016/0010-4655(95)00042-E.
DOI
Rodríguez D, Gutiérrez-de-Terán H. Characterization of the homodimerization interface and functional hotspots of the CXCR4 chemokine receptor. Proteins. 2011;80:1919–1928.
PubMed
Jorgensen WL, Maxwell DS, Tirado-Rives J. Development and testing of the OPLS all-atom force field on conformational energetics and properties of organic liquids. J. Am. Chem. Soc. 1996;118:11225–11236. doi: 10.1021/ja9621760.
DOI
Jorgensen WL, Chandrasekhar J, Madura JD, Impey RW, Klein ML. Comparison of simple potential functions for simulating liquid water. J. Chem. Phys. 1983;79:926–935. doi: 10.1063/1.445869.
DOI
Hess B, Bekker H, Berendsen HJ, Fraaije JG. LINCS: a linear constraint solver for molecular simulations. J. Comput. Chem. 1997;18:1463–1472. doi: 10.1002/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H.
DOI
Miyamoto S, Kollman PA. SETTLE: an analytical version of the SHAKE and RATTLE algorithm for rigid water models. J. Comput. Chem. 1992;13:952–962. doi: 10.1002/jcc.540130805.
DOI
Chakrabarti N, Neale C, Payandeh J, Pai EF, Pomès R. An iris-like mechanism of pore dilation in the CorA magnesium transport system. Biophys. J. 2010;98:784–792. doi: 10.1016/j.bpj.2009.11.009.
PubMed
DOI
PMC
Lindahl E, Edholm O. Mesoscopic undulations and thickness fluctuations in lipid bilayers from molecular dynamics simulations. Biophys. J. 2000;79:426–433. doi: 10.1016/S0006-3495(00)76304-1.
PubMed
DOI
PMC
Berger O, Edholm O, Jähnig F. Molecular dynamics simulations of a fluid bilayer of dipalmitoylphosphatidylcholine at full hydration, constant pressure, and constant temperature. Biophys. J. 1997;72:2002. doi: 10.1016/S0006-3495(97)78845-3.
PubMed
DOI
PMC
Darden T, York D, Pedersen L. Particle mesh Ewald: an N log (N) method for Ewald sums in large systems. J. Chem. Phys. 1993;98:10089–10092. doi: 10.1063/1.464397.
DOI
Nosé S, Klein ML. Constant pressure molecular dynamics for molecular systems. Mol. Phys. 1983;50:1055–1076. doi: 10.1080/00268978300102851.
DOI
Parrinello M, Rahman A. Polymorphic transitions in single crystals: a new molecular dynamics method. J. Appl. Phys. 1981;52:7182–7190. doi: 10.1063/1.328693.
DOI