Overexpression of spoT gene in coccoid forms of clinical Helicobacter pylori isolates
Language English Country United States Media print-electronic
Document type Journal Article
Grant support
390009
Isfahan University of Medical Sciences
PubMed
29327293
DOI
10.1007/s12223-017-0557-0
PII: 10.1007/s12223-017-0557-0
Knihovny.cz E-resources
- MeSH
- Amoxicillin metabolism pharmacology MeSH
- Anti-Bacterial Agents metabolism pharmacology MeSH
- Genes, Bacterial genetics MeSH
- Drug Resistance, Bacterial genetics MeSH
- Adaptation, Physiological genetics MeSH
- Helicobacter pylori drug effects genetics growth & development MeSH
- Microbial Sensitivity Tests MeSH
- Microbial Viability drug effects MeSH
- Pyrophosphatases genetics MeSH
- Gene Expression Regulation, Bacterial drug effects physiology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amoxicillin MeSH
- Anti-Bacterial Agents MeSH
- Pyrophosphatases MeSH
Helicobacter pylori (H. pylori) can convert to coccoid form in unfavorable conditions or as a result of antibiotic treatment. In order to adapt to harsh environments, H. pylori requires a stringent response which, encoded by the spoT gene, has a bifunctional enzyme possessing both (p)ppGpp synthetic and degrading activity. Our goal in this study was to compare spoT gene expression in spiral and induced coccoid forms of H. pylori with use of amoxicillin. First, clinical isolate coccoid forms were induced with amoxicillin; then, the viability test was analyzed by flow cytometer. After RNA extraction, cDNA synthesis and designing a specific primer for spoT gene, evaluation of the desired gene expression in both forms were studied. Bacterial isolates exposed to amoxicillin at MIC and 1/2 MIC induced morphological conversion better and faster than other MIC concentration. The expression of spoT gene was significantly downregulated in spiral forms of H. pylori, while the gene expression was upregulated and + 30.3-fold changes was seen in coccoid forms of bacterium. To summarize, spoT gene is one of the key factors for antibiotic resistance and its enhanced expression in coccoid form can be a valuable diagnostic marker for recognition of H. pylori during morphological conversion.
Department of Microbiology Isfahan University of Medical Sciences Isfahan Iran
Department of Microbiology Tonekabon Branch Islamic Azad University Tonekabon Iran
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