Vagina which is one of the important reservoirs for Staphylococcus and in pregnant women pathogenic strains may infect the child during the birth or by vertical transmission. A total of 68 presumptive Staphylococcus strains isolated from human vagina were found to be gram-positive cocci, and only 32 (47%) isolates were found beta-hemolytic. Matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) results confirmed 33 isolates belonged to Staphylococcus which consisting of 6 species, i.e., S. aureus (14), S. vitulinus (7), S. epidermidis (4), S cohnii (3), S. equorum (3), and S. succinus (2). Further, the result of antibiotic susceptibility tests showed that large proportions (76%-100%) of the isolates were resistant to multiple antibiotics and more often resistant to penicillin (100%), ampicillin (100%), oxacillin (97%), oxytetracycline (97%), vancomycin (97%), rifampin (85%), erythromycin (82%), and streptomycin (76%). In the present study, only the sec enterotoxin gene was detected in four S. aureus strains. DNA fingerprints of the 33 isolates that were generated using random amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) PCR analysis revealed great genetic relatedness of isolates. High prevalence of vaginal colonization with multiple antibiotic-resistant staphylococci among pregnant women was observed which were emerged from the single respective species clones that underwent evolution. The vertical transmission of these multiple antibiotic-resistant Staphylococcus species to the infant is possible; therefore, the findings of this study emphasize the need for regular surveillance of antibiotic-resistant bacterial strains in pregnant women in this area.

Department of Microbiology School of Biological Sciences Faculty of Natural and Agricultural Sciences North West University Private Bag X2046 Mmabatho 2735 South Africa

Food Security and Safety Niche Area Faculty of Natural and Agricultural Sciences North West University Private Bag X2046 Mmabatho 2735 South Africa

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Int J Food Microbiol. 2012 Jul 2;157(2):286-96 PubMed

Clin Infect Dis. 2002 Feb 15;34(4):482-92 PubMed

J Microbiol Immunol Infect. 2005 Aug;38(4):283-8 PubMed

Vet Microbiol. 2009 Feb 16;134(1-2):45-54 PubMed

Am J Obstet Gynecol. 2012 Apr;206(4):329.e1-5 PubMed

Microbiol Res. 2009;164(4):404-10 PubMed

Am J Infect Control. 2010 Feb;38(1):72-4 PubMed

J Clin Microbiol. 2003 Apr;41(4):1434-9 PubMed

J Glob Infect Dis. 2015 Jul-Sep;7(3):97-102 PubMed

Vet Microbiol. 2015 Apr 17;176(3-4):382-8 PubMed

Lancet Infect Dis. 2002 Nov;2(11):677-85 PubMed

Clin Microbiol Rev. 2014 Oct;27(4):870-926 PubMed

Clin Microbiol Rev. 2015 Jul;28(3):603-61 PubMed

Nat Rev Drug Discov. 2013 May;12(5):371-87 PubMed

J Med Microbiol. 2006 Apr;55(Pt 4):387-92 PubMed

Int J Environ Res Public Health. 2015 Aug 25;12(9):10254-75 PubMed

Int J Food Microbiol. 2007 Sep 15;118(2):186-93 PubMed

Eur J Clin Microbiol Infect Dis. 2011 Jun;30(6):719-25 PubMed

Int J Microbiol. 2009;2009:501362 PubMed

Am J Perinatol. 2009 Feb;26(2):145-51 PubMed

J Clin Microbiol. 2002 Jul;40(7):2638-41 PubMed

Proc Biol Sci. 2009 Jul 22;276(1667):2521-30 PubMed

J Med Microbiol. 2006 Feb;55(Pt 2):183-7 PubMed

Paediatr Perinat Epidemiol. 2010 Sep;24(5):488-91 PubMed

Evol Appl. 2015 Mar;8(3):240-7 PubMed

J Pediatric Infect Dis Soc. 2012 Mar;1(1):7-15 PubMed

Scand J Infect Dis. 2011 Dec;43(11-12):930-6 PubMed

J Hosp Infect. 2005 Nov;61(3):225-30 PubMed

Expert Rev Vaccines. 2016 Nov;15(11):1373-1392 PubMed

N Engl J Med. 1998 Aug 20;339(8):520-32 PubMed